Establishment of Orthosiphon stamineus cell suspension culture for cell growth

Wai Leng Lee, Lai Keng Chan

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25 Citations (Scopus)

Abstract

Callus of Orthosiphon stamineus could be induced successfully from petiole, leaf and stem tissues but not roots when cultured on MS medium containing different concentration of NAA (0-4.0 mg l-1) and 2,4-D (0-2.0 mg l-1). Highest fresh weight callus production was obtained from leaf explants and those with best friability were obtained on MS medium plus 1.0 mg l-1 2,4-D plus 1.0 mg l-1 NAA. Cell suspension cultures were established from these cultures. The appropriate cell inoculum size for the best cell growth was 0.75 g of cells in 20 ml culture medium. Cell suspension culture using MS medium supplemented with 1.0 mg l-1 2,4-D promoted the best cell growth with maximum biomass of 8.609 g fresh weight and 0.309 g dry weight 24 days after inoculation. Cells that grew in MS medium supplemented with 1.0 mg l-1 2,4-D reached the stationary growth phase in 15 days as compared to the cells that grew in MS medium supplemented with 1.0 mg l -1 2,4-D+1.0 mgl-1 NAA reached the stationary phase in 24 days. MS medium supplemented with 1.0 mg l-1 2,4-D was considered as the maintenance medium for maintaining the optimum cell growth of 0. stamineus in the cell suspension cultures with 2-week interval subculture.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume78
Issue number2
DOIs
Publication statusPublished - Aug 2004
Externally publishedYes

Keywords

  • Biomass
  • Callus
  • Cell inoculum
  • Cell suspension culture
  • Orthosiphon stamineus

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