Callus of Orthosiphon stamineus could be induced successfully from petiole, leaf and stem tissues but not roots when cultured on MS medium containing different concentration of NAA (0-4.0 mg l-1) and 2,4-D (0-2.0 mg l-1). Highest fresh weight callus production was obtained from leaf explants and those with best friability were obtained on MS medium plus 1.0 mg l-1 2,4-D plus 1.0 mg l-1 NAA. Cell suspension cultures were established from these cultures. The appropriate cell inoculum size for the best cell growth was 0.75 g of cells in 20 ml culture medium. Cell suspension culture using MS medium supplemented with 1.0 mg l-1 2,4-D promoted the best cell growth with maximum biomass of 8.609 g fresh weight and 0.309 g dry weight 24 days after inoculation. Cells that grew in MS medium supplemented with 1.0 mg l-1 2,4-D reached the stationary growth phase in 15 days as compared to the cells that grew in MS medium supplemented with 1.0 mg l -1 2,4-D+1.0 mgl-1 NAA reached the stationary phase in 24 days. MS medium supplemented with 1.0 mg l-1 2,4-D was considered as the maintenance medium for maintaining the optimum cell growth of 0. stamineus in the cell suspension cultures with 2-week interval subculture.
|Number of pages||6|
|Journal||Plant Cell, Tissue and Organ Culture|
|Publication status||Published - Aug 2004|
- Cell inoculum
- Cell suspension culture
- Orthosiphon stamineus