Establishment of Endoderm Progenitors by SOX Transcription Factor Expression in Human Embryonic Stem Cells

Cheryle A. Séguin, Jonathan S. Draper, Andras Nagy, Janet Rossant

Research output: Contribution to journalArticleResearchpeer-review

143 Citations (Scopus)

Abstract

In this study, we explore endoderm cell fate regulation through the expression of lineage-determining transcription factors. We demonstrate that stable endoderm progenitors can be established from human ES cells by constitutive expression of SOX7 or SOX17, producing extraembryonic endoderm and definitive endoderm progenitors, respectively. In teratoma assays and growth factor-mediated differentiation, SOX7 cells appear restricted to the extraembryonic endoderm, and SOX17 cells demonstrate a mesendodermal phenotype in teratomas and the ability to undergo endoderm maturation in vitro in the absence of cytokine-mediated endoderm induction. These endoderm progenitor cells maintain a stable phenotype through many passages in culture, thereby providing new tools to explore the pathways of endoderm differentiation.

Original languageEnglish
Pages (from-to)182-195
Number of pages14
JournalCell Stem Cell
Volume3
Issue number2
DOIs
Publication statusPublished - 7 Aug 2008
Externally publishedYes

Keywords

  • STEMCELL

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