TY - JOUR
T1 - ER membrane bending proteins are necessary for de novo nuclear pore formation
AU - Dawson, T
AU - Lazarus, Michelle
AU - Hetzer, Martin
AU - Wente, Susan
PY - 2009
Y1 - 2009
N2 - Nucleocytoplasmic transport occurs exclusively through nuclear pore complexes (NPCs) embedded in pores formed by inner and outer nuclear membrane fusion. The mechanism for de novo pore and NPC biogenesis remains unclear. Reticulons (RTNs) and Yop1/DP1 are conserved membrane protein families required to form and maintain the tubular endoplasmic reticulum (ER) and the postmitotic nuclear envelope. In this study, we report that members of the RTN and Yop1/DP1 families are required for nuclear pore formation. Analysis of Saccharomyces cerevisiae prp20-G282S and nup133Δ NPC assembly mutants revealed perturbations in Rtn1 - green fl uorescent protein (GFP) and Yop1-GFP ER distribution and colocalization to NPC clusters. Combined deletion of RTN1 and YOP1 resulted in NPC clustering, nuclear import defects, and synthetic lethality with the additional absence of Pom34, Pom152, and Nup84 subcomplex members. We tested for a direct role in NPC biogenesis using Xenopus laevis in vitro assays and found that anti-Rtn4a antibodies specifi cally inhibited de novo nuclear pore formation. We hypothesize that these ER membrane - bending proteins mediate early NPC assembly steps.
AB - Nucleocytoplasmic transport occurs exclusively through nuclear pore complexes (NPCs) embedded in pores formed by inner and outer nuclear membrane fusion. The mechanism for de novo pore and NPC biogenesis remains unclear. Reticulons (RTNs) and Yop1/DP1 are conserved membrane protein families required to form and maintain the tubular endoplasmic reticulum (ER) and the postmitotic nuclear envelope. In this study, we report that members of the RTN and Yop1/DP1 families are required for nuclear pore formation. Analysis of Saccharomyces cerevisiae prp20-G282S and nup133Δ NPC assembly mutants revealed perturbations in Rtn1 - green fl uorescent protein (GFP) and Yop1-GFP ER distribution and colocalization to NPC clusters. Combined deletion of RTN1 and YOP1 resulted in NPC clustering, nuclear import defects, and synthetic lethality with the additional absence of Pom34, Pom152, and Nup84 subcomplex members. We tested for a direct role in NPC biogenesis using Xenopus laevis in vitro assays and found that anti-Rtn4a antibodies specifi cally inhibited de novo nuclear pore formation. We hypothesize that these ER membrane - bending proteins mediate early NPC assembly steps.
UR - http://www.scopus.com/inward/record.url?scp=64749091534&partnerID=8YFLogxK
U2 - 10.1083/jcb.200806174
DO - 10.1083/jcb.200806174
M3 - Article
C2 - 19273614
AN - SCOPUS:64749091534
SN - 0021-9525
VL - 184
SP - 659
EP - 675
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -