Epithelial de-differentiation triggered by co-ordinate epigenetic inactivation of the EHF and CDX1 transcription factors drives colorectal cancer progression

Ian Y. Luk, Laura J. Jenkins, Kael L. Schoffer, Irvin Ng, Janson W.T. Tse, Dmitri Mouradov, Stanislaw Kaczmarczyk, Rebecca Nightingale, Allan D. Burrows, Robin Lesley Anderson, Diego Arango, Higinio Dopeso, Larry Croft, Mark F. Richardson, Oliver M. Sieber, Yang Liao, Jennifer K. Mooi, Natalia Vukelic, Camilla M. Reehorst, Shoukat Afshar-SterleVicki L.J. Whitehall, Lochlan Fennell, Helen E. Abud, Niall C. Tebbutt, Wayne A. Phillips, David S. Williams, Wei Shi, Lisa A. Mielke, Matthias Ernst, Amardeep S. Dhillon, Nicholas J. Clemons, John M. Mariadason

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Colorectal cancers (CRCs) often display histological features indicative of aberrant differentiation but the molecular underpinnings of this trait and whether it directly drives disease progression is unclear. Here, we identify co-ordinate epigenetic inactivation of two epithelial-specific transcription factors, EHF and CDX1, as a mechanism driving differentiation loss in CRCs. Re-expression of EHF and CDX1 in poorly-differentiated CRC cells induced extensive chromatin remodelling, transcriptional re-programming, and differentiation along the enterocytic lineage, leading to reduced growth and metastasis. Strikingly, EHF and CDX1 were also able to reprogramme non-colonic epithelial cells to express colonic differentiation markers. By contrast, inactivation of EHF and CDX1 in well-differentiated CRC cells triggered tumour de-differentiation. Mechanistically, we demonstrate that EHF physically interacts with CDX1 via its PNT domain, and that these transcription factors co-operatively drive transcription of the colonic differentiation marker, VIL1. Compound genetic deletion of Ehf and Cdx1 in the mouse colon disrupted normal colonic differentiation and significantly enhanced colorectal tumour progression. These findings thus reveal a novel mechanism driving epithelial de-differentiation and tumour progression in CRC.

Original languageEnglish
Pages (from-to)2288–2302
Number of pages15
JournalCell Death and Differentiation
Volume29
Issue number11
DOIs
Publication statusPublished - Nov 2022

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