Epigenetic regulation of multidrug resistance 1 gene expression: profiling CpG methylation status using bisulphite sequencing.

Emma K Baker, Assam El-Osta

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

21 Citations (Scopus)

Abstract

Methylation of CpG dinucleotides is one of the major epigenetic processes involved in the regulation of gene expression. Catalyzed by DNA methyltransferases, hypermethylation of CpG islands in promoter regions is typically associated with gene silencing. DNA methylation plays an important role in normal differentiation, development, and maintenance of genomic stability, with aberrant CpG methylation being linked with a number of disease states. Three CpG islands within a 1.15- kb region characterize the chromatin landscape surrounding the transcriptional start site of the multidrug resistance 1 (MDR1) gene. We and others have demonstrated that hypermethylation of this region is correlated with MDR1 gene silencing and the inability of chemotherapeutic agents to activate MDR1 transcription. The bisulphite sequencing and cloning method allows a precise interpretation of the methylation status of each individual CpG dinucleotide in the MDR1 region.

Original languageEnglish
Title of host publicationMulti-Drug Resistance in Cancer
EditorsJun Zhou
Place of PublicationTotowa, NJ USA
PublisherHumana Press
Chapter9
Pages183-198
Number of pages16
Edition1st
ISBN (Print)9781607614159
Publication statusPublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume596
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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