Enzyme activity assays within microstructured optical fibers enabled by automated alignment

Stephen C. Warren-Smith; Guiying Nie; Erik P. Schartner; Lois A. Salamonsen; Tanya M. Monro

doi:10.1364/BOE.3.003304

Enzyme activity assays within microstructured optical fibers enabled by automated alignment

Stephen C. Warren-Smith
, Guiying Nie
, Erik P. Schartner
, Lois A. Salamonsen
, Tanya M. Monro

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women's health.

Original language	English
Pages (from-to)	3304-3313
Number of pages	10
Journal	Biomedical Optics Express
Volume	3
Issue number	12
DOIs	https://doi.org/10.1364/BOE.3.003304
Publication status	Published - 1 Dec 2012
Externally published	Yes

Keywords

Fiber optics sensors
Microstructured fibers
Biological sensing and sensors

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10.1364/BOE.3.003304

Cite this

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abstract = "A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women's health.",

keywords = "Fiber optics sensors, Microstructured fibers, Biological sensing and sensors",

author = "Warren-Smith, \{Stephen C.\} and Guiying Nie and Schartner, \{Erik P.\} and Salamonsen, \{Lois A.\} and Monro, \{Tanya M.\}",

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AU - Nie, Guiying

AU - Schartner, Erik P.

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AU - Monro, Tanya M.

PY - 2012/12/1

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AB - A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women's health.

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KW - Microstructured fibers

KW - Biological sensing and sensors

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Enzyme activity assays within microstructured optical fibers enabled by automated alignment

Abstract

Keywords

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Focus on the uterus: basic and translational researchstrategies to address key issues in women's health. Fertility, infertility, endometrial and pregnancy disorders

Cite this

Enzyme activity assays within microstructured optical fibers enabled by automated alignment

Abstract

Keywords

Access to Document

Other files and links

Projects

Focus on the uterus: basic and translational researchstrategies to address key issues in women's health. Fertility, infertility, endometrial and pregnancy disorders

Cite this