Enzyme activity assays within microstructured optical fibers enabled by automated alignment

Stephen C. Warren-Smith, Guiying Nie, Erik P. Schartner, Lois A. Salamonsen, Tanya M. Monro

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

A fluorescence-based enzyme activity assay has been demonstrated within a small-core microstructured optical fiber (MOF) for the first time. To achieve this, a reflection-based automated alignment system has been developed, which uses feedback and piezoelectric actuators to maintain optical alignment. The auto-alignment system provides optical stability for the time required to perform an activity assay. The chosen assay is based on the enzyme proprotein convertase 5/6 (PC6) and has important applications in women's health.

Original languageEnglish
Pages (from-to)3304-3313
Number of pages10
JournalBiomedical Optics Express
Volume3
Issue number12
DOIs
Publication statusPublished - 1 Dec 2012
Externally publishedYes

Keywords

  • Fiber optics sensors
  • Microstructured fibers
  • Biological sensing and sensors

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