Enzymatic U(VI) reduction by Desulfosporosinus species

Yohey Suzuki; Shelly D. Kelly; Kenneth M. Kemner; Jillian F. Banfield

doi:10.1524/ract.92.1.11.25404

Enzymatic U(VI) reduction by Desulfosporosinus species

Yohey Suzuki, Shelly D. Kelly, Kenneth M. Kemner, Jillian F. Banfield

Research output: Contribution to journal › Article › Research › peer-review

67 Citations (Scopus)

Abstract

Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1 mM U(VI) and lactate, under an atmosphere containing N₂-CO ₂-H₂ (90: 5: 5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25% Na-bicarbonate or 0.85% NaCl. U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfurìcans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H₂ electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1 mM CuCl₂ was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c₃ used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway.

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Radiochimica Acta
Volume	92
Issue number	1
DOIs	https://doi.org/10.1524/ract.92.1.11.25404
Publication status	Published - 2004
Externally published	Yes

Keywords

Desulfosporosinus
Gram-positive
Spore-forming
Sulfate-reducing bacteria
Uranium reduction

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10.1524/ract.92.1.11.25404

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@article{816121d521c246829c30a15257b1e107,

title = "Enzymatic U(VI) reduction by Desulfosporosinus species",

abstract = "Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1 mM U(VI) and lactate, under an atmosphere containing N2-CO 2-H2 (90: 5: 5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25\% Na-bicarbonate or 0.85\% NaCl. U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfur{\`i}cans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H2 electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1 mM CuCl2 was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c3 used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway.",

keywords = "Desulfosporosinus, Gram-positive, Spore-forming, Sulfate-reducing bacteria, Uranium reduction",

author = "Yohey Suzuki and Kelly, \{Shelly D.\} and Kemner, \{Kenneth M.\} and Banfield, \{Jillian F.\}",

note = "Funding Information: Acknowledgment. This research was supported by grants from the U.S. Department of Energy, Basic Energy Science Program and the U.S. Department of Energy, Biological and Environmental Research (NABIR) Program. YS is grateful to Yoshida Scholarship for additional financial support. Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract No. W-31-109-Eng-38. Work performed at the MRCAT beamline is supported, in part, by funding from the Dept. of Energy under grant number DEFG2000ER45811. The authors thank Donna Bruce and Ellie Hale for assistance with sample collection. We acknowledge Edward O{\textquoteright}Loughlin for his assistance in collecting the XANES data. We are grateful to Jorge Escalante, Vince Starai, and Matthias Labrenz for laboratory assistance.",

year = "2004",

doi = "10.1524/ract.92.1.11.25404",

language = "English",

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pages = "11--16",

journal = "Radiochimica Acta",

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T1 - Enzymatic U(VI) reduction by Desulfosporosinus species

AU - Suzuki, Yohey

AU - Kelly, Shelly D.

AU - Kemner, Kenneth M.

AU - Banfield, Jillian F.

N1 - Funding Information: Acknowledgment. This research was supported by grants from the U.S. Department of Energy, Basic Energy Science Program and the U.S. Department of Energy, Biological and Environmental Research (NABIR) Program. YS is grateful to Yoshida Scholarship for additional financial support. Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract No. W-31-109-Eng-38. Work performed at the MRCAT beamline is supported, in part, by funding from the Dept. of Energy under grant number DEFG2000ER45811. The authors thank Donna Bruce and Ellie Hale for assistance with sample collection. We acknowledge Edward O’Loughlin for his assistance in collecting the XANES data. We are grateful to Jorge Escalante, Vince Starai, and Matthias Labrenz for laboratory assistance.

PY - 2004

Y1 - 2004

N2 - Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1 mM U(VI) and lactate, under an atmosphere containing N2-CO 2-H2 (90: 5: 5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25% Na-bicarbonate or 0.85% NaCl. U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfurìcans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H2 electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1 mM CuCl2 was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c3 used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway.

AB - Here we tested U(VI) reduction by a Desulfosporosinus species (sp.) isolate and type strain (DSM 765) in cell suspensions (pH 7) containing 1 mM U(VI) and lactate, under an atmosphere containing N2-CO 2-H2 (90: 5: 5). Although neither Desulfosporosinus species (spp.) reduced U(VI) in cell suspensions with 0.25% Na-bicarbonate or 0.85% NaCl. U(VI) was reduced in these solutions by a control strain, Desulfovibrio desulfurìcans (ATCC 642). However, both Desulfosporosinus strains reduced U(VI) in cell suspensions depleted in bicarbonate and NaCl. No U(VI) reduction was observed without lactate and H2 electron donors or with heat-killed cells, indicating enzymatic U(VI) reduction. Uranium(VI) reduction by both strains was inhibited when 1 mM CuCl2 was added to the cell suspensions. Because the Desulfosporosinus DSM 765 does not contain cytochrome c3 used by Desulfovibrio spp. to reduce U(VI), Desulfosporosinus species reduce uranium via a different enzymatic pathway.

KW - Desulfosporosinus

KW - Gram-positive

KW - Spore-forming

KW - Sulfate-reducing bacteria

KW - Uranium reduction

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DO - 10.1524/ract.92.1.11.25404

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AN - SCOPUS:0842269099

SN - 0033-8230

VL - 92

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EP - 16

JO - Radiochimica Acta

JF - Radiochimica Acta

IS - 1

ER -

Enzymatic U(VI) reduction by Desulfosporosinus species

Abstract

Keywords

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