TY - JOUR
T1 - Enzymatic rotating biosensor for ciprofloxacin determination
AU - Torriero, Angel Alberto Jesus
AU - Ruiz-Diaz, Juan J J
AU - Salinas, Eloy
AU - Marchevsky, Eduardo
AU - Sanz-Ferramola, Maria Isabel
AU - Raba, Julio
PY - 2006
Y1 - 2006
N2 - The high sensitivity that can be attained using an enzymic system and mediated by catechol was verified by online interfacing of a rotating biosensor and continuous flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide, catalyzed the oxidn. of catechol, whose back electrochem. redn. was detected on a glassy carbon electrode surface at -200 mV. Thus, when ciprofloxacin (CF) was added to the soln., this piperazine-contg. compd. participate in Michael addn. reactions with catechol to form the corresponding piperazine-quinone derivs., decreasing the peak current obtained, in proportion with the increase of its concn. The highest response for CF was obtained around pH 7. This method could be used to det. CF concn. in the range of 0.02-65 micro M (r = 0.999). The detn. of CF concn. was possible with a detection limit of 0.4 nM, in the processing of as many as 25 samples per h. Application of this anal. to different pharmaceutical samples contg. CF supports the utility of the HRP-rotating biosensor. [on SciFinder (R)]
AB - The high sensitivity that can be attained using an enzymic system and mediated by catechol was verified by online interfacing of a rotating biosensor and continuous flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide, catalyzed the oxidn. of catechol, whose back electrochem. redn. was detected on a glassy carbon electrode surface at -200 mV. Thus, when ciprofloxacin (CF) was added to the soln., this piperazine-contg. compd. participate in Michael addn. reactions with catechol to form the corresponding piperazine-quinone derivs., decreasing the peak current obtained, in proportion with the increase of its concn. The highest response for CF was obtained around pH 7. This method could be used to det. CF concn. in the range of 0.02-65 micro M (r = 0.999). The detn. of CF concn. was possible with a detection limit of 0.4 nM, in the processing of as many as 25 samples per h. Application of this anal. to different pharmaceutical samples contg. CF supports the utility of the HRP-rotating biosensor. [on SciFinder (R)]
UR - http://www.sciencedirect.com.ezproxy.lib.monash.edu.au/science?_ob=MImg&_imagekey=B6THP-4HTBKTC-1-Y&_cdi=5288&_user=542840&_pii=S0039914005007332&_ori
M3 - Article
SN - 0039-9140
VL - 69
SP - 691
EP - 699
JO - Talanta
JF - Talanta
IS - 3
ER -