TY - JOUR
T1 - Enhancing the reusability of endoglucanase-gold nanoparticle bioconjugates by tethering to polyurethane microspheres
AU - Phadtare, Sumant
AU - Vyas, Santosh
AU - Palaskar, Dnyaneshwar V.
AU - Lachke, Anil
AU - Shukla, Parshuram G.
AU - Sivaram, Swaminathan
AU - Sastry, Murali
PY - 2004/11
Y1 - 2004/11
N2 - The synthesis of polyurethane microsphere-gold nanoparticle "core-shell" structures and their use in the immobilization of the enzyme endoglucanase are described. Assembly of gold nanoparticles on the surface of polymer microspheres occurs through interaction of the nitrogens in the polymer with the nanoparticles, thereby precluding the need for modifying the polymer microspheres to enable such nanoparticle binding. Endoglucanse could thereafter be bound to the gold nanoparticles decorating the polyurethane microspheres, leading to a highly stable biocatalyst with excellent reuse characteristics. The immobilized enzyme retains its biocatalytic activity and exhibits improved thermal stability relative to free enzyme in solution. The high surface area of the host gold nanoparticles renders the immobilized enzyme "quasi free", while at the same time retaining advantages of immobilization such as ease of reuse, enhanced temporal and thermal stability, etc.
AB - The synthesis of polyurethane microsphere-gold nanoparticle "core-shell" structures and their use in the immobilization of the enzyme endoglucanase are described. Assembly of gold nanoparticles on the surface of polymer microspheres occurs through interaction of the nitrogens in the polymer with the nanoparticles, thereby precluding the need for modifying the polymer microspheres to enable such nanoparticle binding. Endoglucanse could thereafter be bound to the gold nanoparticles decorating the polyurethane microspheres, leading to a highly stable biocatalyst with excellent reuse characteristics. The immobilized enzyme retains its biocatalytic activity and exhibits improved thermal stability relative to free enzyme in solution. The high surface area of the host gold nanoparticles renders the immobilized enzyme "quasi free", while at the same time retaining advantages of immobilization such as ease of reuse, enhanced temporal and thermal stability, etc.
UR - http://www.scopus.com/inward/record.url?scp=10044279271&partnerID=8YFLogxK
U2 - 10.1021/bp0499000
DO - 10.1021/bp0499000
M3 - Article
C2 - 15575720
AN - SCOPUS:10044279271
VL - 20
SP - 1840
EP - 1846
JO - Biotechnology Progress
JF - Biotechnology Progress
SN - 8756-7938
IS - 6
ER -