恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率

Translated title of the contribution: Enhancing expression efficiency of alkaline polygalacturonate lyase by constant cell concentration culture of the recombinant Pichia pastoris

Huilin Wang, Jianghua Li, Long Liu, Jiangning Song, Guocheng Du

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In order to enhance the alkaline polygalacturonate lyase (PGL) productivity by Pichia pastoris, we developed a constant cell concentration culture strategy by methanol feeding (called as CCCM culture) used in the continuous cultures. We controlled reasonable cell concentrations in the bioprocess by different strategies of methanol feeding. Using this CCCM culture with DCW 75 g/L, we significantly enhanced the PGL productivity (QV) and the average specific enzyme production rate (QX) of PGL to 6.11 U/(mL·h) and 81.5 U/(g·h), increased by 42.1% and 191.2% than the fed-batch culture with high cell density, respectively. The final PGL activity was 441.9 U/mL. Moreover, the extracellular protease concentration is 1.9 mg/L and the cell viability is more than 94% after 120 hour induction. The results show that this new strategy is advantageous in reducing proteolytic degradation and enhancing cell viability.

Translated title of the contributionEnhancing expression efficiency of alkaline polygalacturonate lyase by constant cell concentration culture of the recombinant Pichia pastoris
Original languageChinese
Pages (from-to)937-949
Number of pages13
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume28
Issue number8
Publication statusPublished - Aug 2012
Externally publishedYes

Keywords

  • Alkaline polygalacturonate lyase (PGL)
  • CCCM culture
  • Cell concentration
  • Fed-batch culture
  • Production intensity
  • Recombinant Pichia pastoris

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