Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells

Sun Li Chong, Duen Gang Mou, Saw Hoon Lim, Abdul Manaf Ali, Beng Ti Tey

Research output: Contribution to journalArticleResearchpeer-review


BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C(2)E(7) was investigated. Hybridoma growth under hypothermic conditions (32 degrees C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30 ). RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30 FBS supplemented, 32 degrees C) were 1.38- and 1.34-fold greater than the control culture (10 FBS supplemented, 37 degrees C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth. CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells.
Original languageEnglish
Pages (from-to)1674 - 1680
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Issue number11
Publication statusPublished - 2009
Externally publishedYes

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