Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

Romiza Rehana Mazid, Alexandra Claire Cooper, Ying Zhang, Vijayaraghavan Ranganathan, Douglas Robert Macfarlane, Christina Cortez-Jugo, Wenlong Cheng

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).
Original languageEnglish
Pages (from-to)43839 - 43844
Number of pages6
JournalRSC Advances
Volume5
Issue number54
DOIs
Publication statusPublished - 2015

Cite this

Mazid, R. R., Cooper, A. C., Zhang, Y., Ranganathan, V., Macfarlane, D. R., Cortez-Jugo, C., & Cheng, W. (2015). Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids. RSC Advances, 5(54), 43839 - 43844. https://doi.org/10.1039/c5ra05518k
Mazid, Romiza Rehana ; Cooper, Alexandra Claire ; Zhang, Ying ; Ranganathan, Vijayaraghavan ; Macfarlane, Douglas Robert ; Cortez-Jugo, Christina ; Cheng, Wenlong. / Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids. In: RSC Advances. 2015 ; Vol. 5, No. 54. pp. 43839 - 43844.
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abstract = "Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).",
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Mazid, RR, Cooper, AC, Zhang, Y, Ranganathan, V, Macfarlane, DR, Cortez-Jugo, C & Cheng, W 2015, 'Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids' RSC Advances, vol. 5, no. 54, pp. 43839 - 43844. https://doi.org/10.1039/c5ra05518k

Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids. / Mazid, Romiza Rehana; Cooper, Alexandra Claire; Zhang, Ying; Ranganathan, Vijayaraghavan; Macfarlane, Douglas Robert; Cortez-Jugo, Christina; Cheng, Wenlong.

In: RSC Advances, Vol. 5, No. 54, 2015, p. 43839 - 43844.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Mazid, Romiza Rehana

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AU - Cortez-Jugo, Christina

AU - Cheng, Wenlong

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AB - Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).

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Mazid RR, Cooper AC, Zhang Y, Ranganathan V, Macfarlane DR, Cortez-Jugo C et al. Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids. RSC Advances. 2015;5(54):43839 - 43844. https://doi.org/10.1039/c5ra05518k