Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

Romiza Rehana Mazid; Alexandra Claire Cooper; Ying Zhang; Vijayaraghavan Ranganathan; Douglas Robert Macfarlane; Christina Cortez-Jugo; Wenlong Cheng

doi:10.1039/c5ra05518k

Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

Romiza Rehana Mazid, Alexandra Claire Cooper, Ying Zhang, Vijayaraghavan Ranganathan, Douglas Robert Macfarlane, Christina Cortez-Jugo, Wenlong Cheng

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).

Original language	English
Pages (from-to)	43839 - 43844
Number of pages	6
Journal	RSC Advances
Volume	5
Issue number	54
DOIs	https://doi.org/10.1039/c5ra05518k
Publication status	Published - 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1039/c5ra05518k

Cite this

@article{06b3e1d4c5e94e1083bc0715bee1eede,

title = "Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids",

abstract = "Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).",

author = "Mazid, {Romiza Rehana} and Cooper, {Alexandra Claire} and Ying Zhang and Vijayaraghavan Ranganathan and Macfarlane, {Douglas Robert} and Christina Cortez-Jugo and Wenlong Cheng",

year = "2015",

doi = "10.1039/c5ra05518k",

language = "English",

volume = "5",

pages = "43839 -- 43844",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "The Royal Society of Chemistry",

number = "54",

}

TY - JOUR

T1 - Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

AU - Mazid, Romiza Rehana

AU - Cooper, Alexandra Claire

AU - Zhang, Ying

AU - Ranganathan, Vijayaraghavan

AU - Macfarlane, Douglas Robert

AU - Cortez-Jugo, Christina

AU - Cheng, Wenlong

PY - 2015

Y1 - 2015

N2 - Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).

AB - Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pDNA was maintained under such conditions, as demonstrated by the expression of yellow fluorescent protein (YFP).

UR - http://pubs.rsc.org.ezproxy.lib.monash.edu.au/en/content/articlepdf/2015/ra/c5ra05518k

U2 - 10.1039/c5ra05518k

DO - 10.1039/c5ra05518k

M3 - Article

SN - 2046-2069

VL - 5

SP - 43839

EP - 43844

JO - RSC Advances

JF - RSC Advances

IS - 54

ER -

Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

Abstract

UN SDGs

Access to Document

Other files and links

Protonic Materials for Green Chemical Futures

Engineering pore forming proteins as machines for the delivery of proteins and nanoparticles into cells

Cite this

Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids

Abstract

UN SDGs

Access to Document

Other files and links

Projects

Protonic Materials for Green Chemical Futures

Engineering pore forming proteins as machines for the delivery of proteins and nanoparticles into cells

Cite this