Engineering of a bis-chelator motif into a protein alpha-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

James Swarbrick, Phuc Ung, Xun-Cheng Su, Ansis Maleckis, Sandeep Chhabra, Thomas Huber, Gottfried Otting, Bimbil Graham

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38 Citations (Scopus)

Abstract

Attachment of two nitrilotriacetic acid-based ligands to a protein alpha-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.
Original languageEnglish
Pages (from-to)7368 - 7370
Number of pages3
JournalChemical Communications
Volume47
DOIs
Publication statusPublished - 2011

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