Engineering fluorescent poly(dopamine) capsules

Xi Chen, Yan Yan, Markus Mullner, Martin P. Van Koeverden, Ka Fung Noi, Wei Zhu, Frank Caruso

Research output: Contribution to journalArticleResearchpeer-review

88 Citations (Scopus)


The recent development of poly(dopamine) (PDA) capsules provides new opportunities for their application in biology and medicine. To advance the biomedical application of PDA capsules, strategies that enable the preparation of fluorescently labeled PDA (F-PDA) capsules are required, as this will allow evaluation of their cellular interactions using a range of fluorescence-based techniques. Herein, we report a facile approach for the fabrication of F-PDA capsules via the polymerization of dopamine (DA) on sacrificial templates in the presence of hydrogen peroxide (H2O2). F-PDA capsules with well-defined sizes are prepared by templating different organic and inorganic particles. The resulting F-PDA capsules show negligible cytotoxicity in HeLa cells after incubation for 48 h. We also demonstrate visualization of the F-PDA capsules following internalization by HeLa cells using conventional fluorescence microscopy, en route toward detailed investigations on their biological interactions. 

Original languageEnglish
Pages (from-to)2921-2925
Number of pages5
Issue number10
Publication statusPublished - 18 Mar 2014
Externally publishedYes

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