Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Dongbin Jin; Khai Lee Loh; Tima Shamekhi; Yi Tian Ting; Terry C.C. Lim Kam Sian; James Roest; Joshua D. Ooi; Julian P. Vivian; Pouya Faridi

doi:10.1007/978-1-0716-3331-1_25

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Dongbin Jin, Khai Lee Loh, Tima Shamekhi, Yi Tian Ting, Terry C.C. Lim Kam Sian, James Roest, Joshua D. Ooi, Julian P. Vivian, Pouya Faridi

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

Original language	English
Title of host publication	Infammation and Cancer
Subtitle of host publication	Methods and Protocols
Editors	Brendan J. Jenkins
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	25
Pages	351-369
Number of pages	19
Edition	2nd
ISBN (Electronic)	9781071633311
ISBN (Print)	9781071633304
DOIs	https://doi.org/10.1007/978-1-0716-3331-1_25
Publication status	Published - 25 Jun 2023

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	2691
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Cancer
Cell lines
Gene editing
HLA
Immunopeptidomics
Immunotherapy
Lentivirus
Neoantigens
Transduction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-3331-1_25

Cite this

Jin, D., Loh, K. L., Shamekhi, T., Ting, Y. T., Lim Kam Sian, T. C. C., Roest, J., Ooi, J. D., Vivian, J. P., & Faridi, P. (2023). Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. In B. J. Jenkins (Ed.), Infammation and Cancer: Methods and Protocols (2nd ed., pp. 351-369). (Methods in Molecular Biology; Vol. 2691). Humana Press. https://doi.org/10.1007/978-1-0716-3331-1_25

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abstract = "Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.",

keywords = "Cancer, Cell lines, Gene editing, HLA, Immunopeptidomics, Immunotherapy, Lentivirus, Neoantigens, Transduction",

author = "Dongbin Jin and Loh, {Khai Lee} and Tima Shamekhi and Ting, {Yi Tian} and {Lim Kam Sian}, {Terry C.C.} and James Roest and Ooi, {Joshua D.} and Vivian, {Julian P.} and Pouya Faridi",

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Jin, D, Loh, KL, Shamekhi, T, Ting, YT , Lim Kam Sian, TCC, Roest, J, Ooi, JD, Vivian, JP & Faridi, P 2023, Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. in BJ Jenkins (ed.), Infammation and Cancer: Methods and Protocols. 2nd edn, Methods in Molecular Biology, vol. 2691, Humana Press, New York NY USA, pp. 351-369. https://doi.org/10.1007/978-1-0716-3331-1_25

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. / Jin, Dongbin; Loh, Khai Lee; Shamekhi, Tima et al.
Infammation and Cancer: Methods and Protocols. ed. / Brendan J. Jenkins. 2nd. ed. New York NY USA: Humana Press, 2023. p. 351-369 (Methods in Molecular Biology; Vol. 2691).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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T1 - Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

AU - Jin, Dongbin

AU - Loh, Khai Lee

AU - Shamekhi, Tima

AU - Ting, Yi Tian

AU - Lim Kam Sian, Terry C.C.

AU - Roest, James

AU - Ooi, Joshua D.

AU - Vivian, Julian P.

AU - Faridi, Pouya

PY - 2023/6/25

Y1 - 2023/6/25

N2 - Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

AB - Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

KW - Cancer

KW - Cell lines

KW - Gene editing

KW - HLA

KW - Immunopeptidomics

KW - Immunotherapy

KW - Lentivirus

KW - Neoantigens

KW - Transduction

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A2 - Jenkins, Brendan J.

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ER -

Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Abstract

Publication series

Keywords

UN SDGs

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