Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

Dongbin Jin, Khai Lee Loh, Tima Shamekhi, Yi Tian Ting, Terry C.C. Lim Kam Sian, James Roest, Joshua D. Ooi, Julian P. Vivian, Pouya Faridi

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review


Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

Original languageEnglish
Title of host publicationInfammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages19
ISBN (Electronic)9781071633311
ISBN (Print)9781071633304
Publication statusPublished - 25 Jun 2023

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cancer
  • Cell lines
  • Gene editing
  • HLA
  • Immunopeptidomics
  • Immunotherapy
  • Lentivirus
  • Neoantigens
  • Transduction

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