Endotoxin-induced basal respiration alterations of renal HK-2 cells: A sign of pathologic metabolism down-regulation

C. Quoilin, A. Mouithys-Mickalad, J. Duranteau, Bernard Gallez, M. Hoebeke

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14 Citations (Scopus)

Abstract

To study the mechanism of oxygen regulation in inflammation-induced acute kidney injury, we investigate the effects of a bacterial endotoxin (lipopolysaccharide, LPS) on the basal respiration of proximal tubular epithelial cells (HK-2) both by high-resolution respirometry and electron spin resonance spectroscopy. These two complementary methods have shown that HK-2 cells exhibit a decreased oxygen consumption rate when treated with LPS. Surprisingly, this cellular respiration alteration persists even after the stress factor was removed. We suggested that this irreversible decrease in renal oxygen consumption after LPS challenge is related to a pathologic metabolic down-regulation such as a lack of oxygen utilization by cells.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume423
Issue number2
DOIs
Publication statusPublished - 29 Jun 2012
Externally publishedYes

Keywords

  • Acute kidney injury (AKI)
  • Endotoxin
  • Inflammation
  • Metabolism
  • Oxygen consumption
  • Sepsis

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