ENDOTHELIN RECEPTORS IN RAT ADRENAL GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY

Masahiro Kohzuki; Colin I. Johnston; Siew Yeen Chai; David J. Casley; Fraser Rogerson; Frederick A O Mendelsohn

doi:10.1111/j.1440-1681.1989.tb01550.x

ENDOTHELIN RECEPTORS IN RAT ADRENAL GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY

Masahiro Kohzuki, Colin I. Johnston, Siew Yeen Chai, David J. Casley, Fraser Rogerson, Frederick A O Mendelsohn

Research output: Contribution to journal › Article › Research › peer-review

30 Citations (Scopus)

Abstract

1. The radioligand [¹²⁵I]‐endothelin was used to map receptors for endothelin in rat adrenal gland using in vitro autoradiography and computerized densitometry. 2. In the adrenal, a high density of binding was found in the adrenal medulla (binding affinity constant 0.18 ± 0.11 × 10⁹M⁻¹) and zona glomerulosa (binding affinity constant 0.18 ± 0.07 × 10⁹M⁻¹). Binding was low to undetectable in the zona fasciculata and zona reticularis. Unrelated peptides did not displace endothelin. 3. These results provide evidence of endothelin receptor distribution in adrenal gland and suggest that endothelin might exert multiple actions in the adrenal gland on catecholamine and aldosterone biosynthesis and secretion.

Original language	English
Pages (from-to)	239-242
Number of pages	4
Journal	Clinical and Experimental Pharmacology and Physiology
Volume	16
Issue number	4
DOIs	https://doi.org/10.1111/j.1440-1681.1989.tb01550.x
Publication status	Published - 1 Jan 1989
Externally published	Yes

Keywords

adrenal gland
autoradiography
endothelin

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10.1111/j.1440-1681.1989.tb01550.x

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title = "ENDOTHELIN RECEPTORS IN RAT ADRENAL GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY",

abstract = "1. The radioligand [125I]‐endothelin was used to map receptors for endothelin in rat adrenal gland using in vitro autoradiography and computerized densitometry. 2. In the adrenal, a high density of binding was found in the adrenal medulla (binding affinity constant 0.18 ± 0.11 × 109M−1) and zona glomerulosa (binding affinity constant 0.18 ± 0.07 × 109M−1). Binding was low to undetectable in the zona fasciculata and zona reticularis. Unrelated peptides did not displace endothelin. 3. These results provide evidence of endothelin receptor distribution in adrenal gland and suggest that endothelin might exert multiple actions in the adrenal gland on catecholamine and aldosterone biosynthesis and secretion.",

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AU - Johnston, Colin I.

AU - Chai, Siew Yeen

AU - Casley, David J.

AU - Rogerson, Fraser

AU - Mendelsohn, Frederick A O

PY - 1989/1/1

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N2 - 1. The radioligand [125I]‐endothelin was used to map receptors for endothelin in rat adrenal gland using in vitro autoradiography and computerized densitometry. 2. In the adrenal, a high density of binding was found in the adrenal medulla (binding affinity constant 0.18 ± 0.11 × 109M−1) and zona glomerulosa (binding affinity constant 0.18 ± 0.07 × 109M−1). Binding was low to undetectable in the zona fasciculata and zona reticularis. Unrelated peptides did not displace endothelin. 3. These results provide evidence of endothelin receptor distribution in adrenal gland and suggest that endothelin might exert multiple actions in the adrenal gland on catecholamine and aldosterone biosynthesis and secretion.

AB - 1. The radioligand [125I]‐endothelin was used to map receptors for endothelin in rat adrenal gland using in vitro autoradiography and computerized densitometry. 2. In the adrenal, a high density of binding was found in the adrenal medulla (binding affinity constant 0.18 ± 0.11 × 109M−1) and zona glomerulosa (binding affinity constant 0.18 ± 0.07 × 109M−1). Binding was low to undetectable in the zona fasciculata and zona reticularis. Unrelated peptides did not displace endothelin. 3. These results provide evidence of endothelin receptor distribution in adrenal gland and suggest that endothelin might exert multiple actions in the adrenal gland on catecholamine and aldosterone biosynthesis and secretion.

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KW - autoradiography

KW - endothelin

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ER -

ENDOTHELIN RECEPTORS IN RAT ADRENAL GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY

Abstract

Keywords

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