Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma

Joanne Lundy; Daniel Croagh

doi:10.1007/978-1-0716-3331-1_4

Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Patient-derived xenografts (PDXs) are valuable models to study cancer biology, behavior, and response to therapies in vivo. Pancreatic cancer is an aggressive and treatment-resistant disease, and typical biopsies are often of low cellular yield and therefore present challenges for the creation of PDXs. This chapter will describe a method to establish PDX models from tissue biopsies obtained via endoscopic ultrasound-guided fine-needle aspiration, a relatively noninvasive technique which compared to surgery is available to pancreatic cancer patients at all stages of disease. Furthermore, we also describe methods to incorporate “humanization” of PDXs via reconstitution with human immune cells, thus mimicking the immune cell-rich microenvironment of pancreatic tumors.

Original language	English
Title of host publication	Inflammation and Cancer
Subtitle of host publication	Methods and Protocols
Editors	Brendan J. Jenkins
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	4
Pages	43-54
Number of pages	12
Edition	2nd
ISBN (Electronic)	9781071633311
ISBN (Print)	9781071633304
DOIs	https://doi.org/10.1007/978-1-0716-3331-1_4
Publication status	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2691
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

EUS-FNA
EUS-FNB
Humanisation
Inflammation
NOD-SCID mice
Pancreatic cancer
Patient-derived xenograft (PDX)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-3331-1_4

Cite this

Lundy, J., & Croagh, D. (2023). Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma. In B. J. Jenkins (Ed.), Inflammation and Cancer: Methods and Protocols (2nd ed., pp. 43-54). (Methods in Molecular Biology; Vol. 2691). Humana Press. https://doi.org/10.1007/978-1-0716-3331-1_4

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abstract = "Patient-derived xenografts (PDXs) are valuable models to study cancer biology, behavior, and response to therapies in vivo. Pancreatic cancer is an aggressive and treatment-resistant disease, and typical biopsies are often of low cellular yield and therefore present challenges for the creation of PDXs. This chapter will describe a method to establish PDX models from tissue biopsies obtained via endoscopic ultrasound-guided fine-needle aspiration, a relatively noninvasive technique which compared to surgery is available to pancreatic cancer patients at all stages of disease. Furthermore, we also describe methods to incorporate “humanization” of PDXs via reconstitution with human immune cells, thus mimicking the immune cell-rich microenvironment of pancreatic tumors.",

keywords = "EUS-FNA, EUS-FNB, Humanisation, Inflammation, NOD-SCID mice, Pancreatic cancer, Patient-derived xenograft (PDX)",

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Lundy, J & Croagh, D 2023, Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma. in BJ Jenkins (ed.), Inflammation and Cancer: Methods and Protocols. 2nd edn, Methods in Molecular Biology, vol. 2691, Humana Press, New York NY USA, pp. 43-54. https://doi.org/10.1007/978-1-0716-3331-1_4

Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma. / Lundy, Joanne ; Croagh, Daniel.
Inflammation and Cancer: Methods and Protocols. ed. / Brendan J. Jenkins. 2nd. ed. New York NY USA: Humana Press, 2023. p. 43-54 (Methods in Molecular Biology; Vol. 2691).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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Endoscopic Ultrasound-Guided Fine-Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma

Abstract

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Keywords

UN SDGs

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