Endogenous IFN-αβ suppresses colony-stimulating factor (CSF)-1-stimulated macrophage DNA synthesis and mediates inhibitory effects of lipopolysaccharide and TNF-α

John A. Hamilton, Genevieve A. Whitty, Ismail Kola, Paul J. Hertzog

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Abstract

Murine bone marrow-derived macrophages (BMM) are widely used as a suitable model to study the proliferative response to macrophage-CSF or CSF-1. We report here that the amount of DNA synthesis observed in BMM cultures in response to CSF-1 can be masked quite significantly by low levels of IFN-αβ produced in the cultures. It was found that Ab to IFN-αβ could enhance the proliferative response in CSF-treated BMM that were able to respond to endogenous IFN-αβ; however, BMM from mice lacking a component of the type I IFN receptor did not show any enhancement of CSF-1-dependent DNA synthesis on addition of the Ab. While DNA synthesis in CSF-1-stimulated BMM from normal mice was also very sensitive to the inhibitory actions of very low concentrations of added IFN-αβ, DNA synthesis in BMM from the "knockout" mice was not, indicating that the type I IFN receptor component containing the null mutation was essential for signal transduction. Previously it was shown that bacterial LPS, TNF-α, IFN-γ, and cAMP could all inhibit CSF-1-stimulated BMM DNA synthesis and proliferation. Using the combined approach of blocking IFN-αβ Ab and the IFN receptor "knockout" mice, it was found here that the growth-inhibitory effects of LPS and TNF-α are due, to a significant extent, to endogenous IFN-αβ, whereas those of IFN-γ and cAMP occur by a different mechanism. It is proposed that the type I IFN receptor (IFNAR 1) "knockout" mice may be useful in delineating some of the in vivo actions of CSF-1, LPS, TNF-α, and possibly other agents.

Original languageEnglish
Pages (from-to)2553-2557
Number of pages5
JournalJournal of Immunology
Volume156
Issue number7
Publication statusPublished - 1 Apr 1996

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