TY - JOUR
T1 - Encapsulation of Lactobacillus plantarum in porous maize starch
AU - Li, Haiteng
AU - Thuy Ho, Van Thi
AU - Turner, Mark S.
AU - Dhital, Sushil
PY - 2016/12/1
Y1 - 2016/12/1
N2 - This study investigated the survival of probiotic Lactobacillus plantarum 299v microencapsulated in native maize starch or partially hydrolyzed maize starches after acid, bile and heat treatments. Scanning electron microscopy and confocal scanning laser microscopy confirmed that naturally present cavities and channels in native maize starch were enlarged by enzymatic hydrolysis allowing them to be filled with probiotics. The formulations using the modified starches had significantly higher initial viable cells compared to native starch after freeze-drying. Compared to free cells, the microencapsulated probiotic bacteria showed a significant improvement in acid tolerance. When comparing unmodified and modified starches, the enzymatic treatments did not significantly improve relative survival, but did result in significantly higher total probiotic numbers after exposure to acid (pH = 2.0, 1 h), bile salt (3% w/v, 4 h) and heat (60 °C, 15min). These results demonstrate that porous maize starch granules allow for a high probiotic loading efficiency and provide enhanced protection to various stressful conditions compared to free cells.
AB - This study investigated the survival of probiotic Lactobacillus plantarum 299v microencapsulated in native maize starch or partially hydrolyzed maize starches after acid, bile and heat treatments. Scanning electron microscopy and confocal scanning laser microscopy confirmed that naturally present cavities and channels in native maize starch were enlarged by enzymatic hydrolysis allowing them to be filled with probiotics. The formulations using the modified starches had significantly higher initial viable cells compared to native starch after freeze-drying. Compared to free cells, the microencapsulated probiotic bacteria showed a significant improvement in acid tolerance. When comparing unmodified and modified starches, the enzymatic treatments did not significantly improve relative survival, but did result in significantly higher total probiotic numbers after exposure to acid (pH = 2.0, 1 h), bile salt (3% w/v, 4 h) and heat (60 °C, 15min). These results demonstrate that porous maize starch granules allow for a high probiotic loading efficiency and provide enhanced protection to various stressful conditions compared to free cells.
KW - Encapsulation
KW - Lactobacillus plantarum
KW - Modified starch
KW - Prebiotics
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=84989828367&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2016.08.019
DO - 10.1016/j.lwt.2016.08.019
M3 - Article
AN - SCOPUS:84989828367
SN - 0023-6438
VL - 74
SP - 542
EP - 549
JO - LWT
JF - LWT
ER -