Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography

P. J. Hayball; R. L. Nation; F. Bochner; R. K. Le Leu

doi:10.1016/0378-4347(91)80552-N

Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography

P. J. Hayball, R. L. Nation, F. Bochner, R. K. Le Leu

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26 Citations (Scopus)

Abstract

A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).

Original language	English
Pages (from-to)	446-452
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	570
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(91)80552-N
Publication status	Published - 4 Oct 1991

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abstract = "A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).",

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AU - Bochner, F.

AU - Le Leu, R. K.

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N2 - A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).

AB - A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).

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U2 - 10.1016/0378-4347(91)80552-N

DO - 10.1016/0378-4347(91)80552-N

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JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 2

ER -

Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography

Abstract

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