Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography

P. J. Hayball, R. L. Nation, F. Bochner, R. K. Le Leu

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Abstract

A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume570
Issue number2
DOIs
Publication statusPublished - 4 Oct 1991

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