TY - JOUR
T1 - Enantiomeric analysis of amino acids by using comprehensive two-dimensional gas chromatography
AU - Junge, Melanie
AU - Huegel, Helmut
AU - Marriott, Philip
PY - 2007
Y1 - 2007
N2 - The chiral separation of amino acids (AA) derivatised with ethyl chloroformate by using comprehensive two-dimensional gas chromatography is reported. A commercially available enantioselective capillary column (Chirasil-L-Val) has been tested as first-dimension column. Two nonenantioselective stationary phases (BPX50 and BP1) with different column lengths were combined with the enantioselective column, which represent chiral/polar and chiral/low-polarity column sets, respectively. These column sets were evaluated to determine the most useful column combination to provide improved separation efficiency of enantioselective AA analysis. Separations of AA mixtures derivatised either as their N-trifluoroacetyl methyl esters or with methyl chloroformate, performed on a chiral/low-polarity column set, are also shown. The method was demonstrated for chiral analysis of AAs in different beer samples. The major AA in the beer samples was proline with amounts ranging from around 65-95 with minor contents of glycine and the L-enantiomers of alanine, valine, leucine, and isoleucine. Small amounts of D-alanine, at about 1, 1.5, and 15 were detected in the three samples.
AB - The chiral separation of amino acids (AA) derivatised with ethyl chloroformate by using comprehensive two-dimensional gas chromatography is reported. A commercially available enantioselective capillary column (Chirasil-L-Val) has been tested as first-dimension column. Two nonenantioselective stationary phases (BPX50 and BP1) with different column lengths were combined with the enantioselective column, which represent chiral/polar and chiral/low-polarity column sets, respectively. These column sets were evaluated to determine the most useful column combination to provide improved separation efficiency of enantioselective AA analysis. Separations of AA mixtures derivatised either as their N-trifluoroacetyl methyl esters or with methyl chloroformate, performed on a chiral/low-polarity column set, are also shown. The method was demonstrated for chiral analysis of AAs in different beer samples. The major AA in the beer samples was proline with amounts ranging from around 65-95 with minor contents of glycine and the L-enantiomers of alanine, valine, leucine, and isoleucine. Small amounts of D-alanine, at about 1, 1.5, and 15 were detected in the three samples.
UR - http://onlinelibrary.wiley.com.ezproxy.lib.monash.edu.au/doi/10.1002/chir.20371/pdf
U2 - 10.1002/chir.20371
DO - 10.1002/chir.20371
M3 - Article
VL - 19
SP - 228
EP - 234
JO - Chirality
JF - Chirality
SN - 1520-636X
IS - 3
ER -