Emerging purification and isolation

Arijit Nath; Chien Wei Ooi; Sangita K Bhattacharjee; Chiranjib Bhattacharjee

doi:10.1016/B978-0-12-800351-0.00012-2

Emerging purification and isolation

Arijit Nath
, Chien Wei Ooi
, Sangita K Bhattacharjee
, Chiranjib Bhattacharjee

Malaysia School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

1 Citation (Scopus)

Abstract

Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.

Original language	English
Title of host publication	Food Waste Recovery
Subtitle of host publication	Processing Technologies and Industrial Techniques
Editors	Charis M. Galanakis
Place of Publication	London UK
Publisher	Elsevier
Chapter	12
Pages	273-292
Number of pages	20
Edition	1st
ISBN (Print)	9780128003510
DOIs	https://doi.org/10.1016/B978-0-12-800351-0.00012-2
Publication status	Published - 2015

Access to Document

10.1016/B978-0-12-800351-0.00012-2

1 Citations
1 Chapter (Book)

Emerging purification and isolation
Nath, A., Ooi, C. W., Bhattacharjee, S. & Bhattacharjee, C., 2021, Food Waste Recovery: Processing Technologies, Industrial Techniques, and Applications. Galankis, C. M. (ed.). 2nd ed. London UK: Elsevier, p. 241-255 15 p.
Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Cite this

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title = "Emerging purification and isolation",

abstract = "Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.",

author = "Arijit Nath and Ooi, \{Chien Wei\} and Bhattacharjee, \{Sangita K\} and Chiranjib Bhattacharjee",

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AB - Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.

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