Emerging purification and isolation

Arijit Nath, Chien Wei Ooi, Sangita K Bhattacharjee, Chiranjib Bhattacharjee

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

    1 Citation (Scopus)

    Abstract

    Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.
    Original languageEnglish
    Title of host publicationFood Waste Recovery: Processing Technologies and Industrial Techniques
    EditorsCharis Michael Galanakis
    Place of PublicationLondon UK
    PublisherElsevier
    Pages273 - 292
    Number of pages20
    Edition1st
    ISBN (Print)9780128003510
    DOIs
    Publication statusPublished - 2015

    Cite this

    Nath, A., Ooi, C. W., Bhattacharjee, S. K., & Bhattacharjee, C. (2015). Emerging purification and isolation. In C. M. Galanakis (Ed.), Food Waste Recovery: Processing Technologies and Industrial Techniques (1st ed., pp. 273 - 292). London UK: Elsevier. https://doi.org/10.1016/B978-0-12-800351-0.00012-2
    Nath, Arijit ; Ooi, Chien Wei ; Bhattacharjee, Sangita K ; Bhattacharjee, Chiranjib. / Emerging purification and isolation. Food Waste Recovery: Processing Technologies and Industrial Techniques. editor / Charis Michael Galanakis. 1st. ed. London UK : Elsevier, 2015. pp. 273 - 292
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    author = "Arijit Nath and Ooi, {Chien Wei} and Bhattacharjee, {Sangita K} and Chiranjib Bhattacharjee",
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    Nath, A, Ooi, CW, Bhattacharjee, SK & Bhattacharjee, C 2015, Emerging purification and isolation. in CM Galanakis (ed.), Food Waste Recovery: Processing Technologies and Industrial Techniques. 1st edn, Elsevier, London UK, pp. 273 - 292. https://doi.org/10.1016/B978-0-12-800351-0.00012-2

    Emerging purification and isolation. / Nath, Arijit; Ooi, Chien Wei; Bhattacharjee, Sangita K; Bhattacharjee, Chiranjib.

    Food Waste Recovery: Processing Technologies and Industrial Techniques. ed. / Charis Michael Galanakis. 1st. ed. London UK : Elsevier, 2015. p. 273 - 292.

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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    AB - Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.

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    Nath A, Ooi CW, Bhattacharjee SK, Bhattacharjee C. Emerging purification and isolation. In Galanakis CM, editor, Food Waste Recovery: Processing Technologies and Industrial Techniques. 1st ed. London UK: Elsevier. 2015. p. 273 - 292 https://doi.org/10.1016/B978-0-12-800351-0.00012-2