Being the fourth step of the 5-Stage Universal Recovery Process, isolation and purification of target compounds become more compact, environmental friendly, and cost effective, producing pure end-products. This chapter presents some emerging purification and isolation techniques, such as, magnetic fishing, aqueous two-phase system, and ion-exchange membrane chromatography. In the presence of magnetic particles, solutes are separated due to their selective affinity and recovered by suitable buffer. On the other hand, the aqueous two-phase system is a liquid/liquid partitioning technique, comprising two types of phase-forming components. Finally, Ion-exchange membrane chromatography is a technique where biomolecules (mostly protein) are separated due to their ionic charge (adsorbtion and elusion by pH of buffer). The features of these technologies are dsecribed in detail in this chapter.
|Title of host publication||Food Waste Recovery: Processing Technologies and Industrial Techniques|
|Editors||Charis Michael Galanakis|
|Place of Publication||London UK|
|Pages||273 - 292|
|Number of pages||20|
|Publication status||Published - 2015|