Emergence of polymyxin B resistance influences pathogenicity in Pseudomonas aeruginosa mutators

Zackery P Bulman, Mark D Sutton, Neang S Ly, Jurgen Bernd Bulitta, Patricia N Holden, Roger Leigh Nation, Jian Li, Brian Tsuji

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

The interplay between polymyxin B pharmacodynamics and pathogenicity was examined in Pseudomonas aeruginosa PAO1 and isogenic DNA repair-deficient mutators (mutM and mutS strains). Against mutS mutators, polymyxin B initial killing was concentration dependent, with >99.9 bacterial reduction at 2 h followed by regrowth and resistance. The pre- versus postexposed strains were inoculated real time into Galleria mellonella waxworms, resulting in increased median survival times from 20 h to 23 h(P <0.001). Emergence of resistance in mutS P. aeruginosa resulted in attenuation of virulence.
Original languageEnglish
Pages (from-to)4343 - 4346
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number7
DOIs
Publication statusPublished - 2015

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