Embryonic toxin expression in the cone snail conus victoriae - primed to kill or divergent function?

Helena Safavi-Hemami, William A Siero, Zhihe Kuang, Nicholas A Williamson, John A Karas, Louise R Page, David MacMillan, Shiva Nag Kompella, David John Adams, Raymond S Norton, Anthony W Purcell

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20 Citations (Scopus)

Abstract

Predatory marine cone snails (genus Conus) utilise complex venoms mainly comprised of small peptide toxins that target voltage- and ligand-gated ion channels in their prey. Whilst the venoms of a number of cone snail species have been intensively profiled and functionally characterised, nothing is known about the initiation of venom expression at an early developmental stage. Here, we report on the expression of venom mRNA in embryos of Conus victoriae and the identification of novel I?- and O-conotoxin sequences. Embryonic toxin mRNA expression is initiated well before differentiation of the venom gland, the organ of venom biosynthesis. Structural and functional studies revealed that the embryonic I?-conotoxins exhibit the same basic three-dimensional structure as the most abundant adult toxin but significantly differ in their neurological targets. Based on these findings, we postulate that the venom repertoire of cone snails undergoes ontogenetic changes most likely reflecting differences in the biotic interactions of these animals with their prey, predators or competitors. To our knowledge, this is the first study to show toxin mRNA transcripts in embryos, a finding that extends our understanding of the early onset of venom expression in animals and may suggest alternative functions of peptide toxins during development.
Original languageEnglish
Pages (from-to)22546 - 22557
Number of pages12
JournalThe Journal of Biological Chemistry
Volume286
Issue number25
DOIs
Publication statusPublished - 2011

Cite this

Safavi-Hemami, H., Siero, W. A., Kuang, Z., Williamson, N. A., Karas, J. A., Page, L. R., MacMillan, D., Kompella, S. N., Adams, D. J., Norton, R. S., & Purcell, A. W. (2011). Embryonic toxin expression in the cone snail conus victoriae - primed to kill or divergent function? The Journal of Biological Chemistry, 286(25), 22546 - 22557. https://doi.org/10.1074/jbc.M110.217703