Embryonic stem cell-somatic cell fusion and postfusion enucleation

Huseyin Sumer; Paul J. Verma

doi:10.1007/978-1-4939-1594-1_5

Embryonic stem cell-somatic cell fusion and postfusion enucleation

Huseyin Sumer, Paul J. Verma

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.

Original language	English
Title of host publication	Nuclear Reprogramming
Subtitle of host publication	Methods and Protocols
Place of Publication	NY USA
Publisher	Humana Press
Chapter	5
Pages	61-69
Number of pages	9
Volume	1222
Edition	2nd
ISBN (Electronic)	9781493915941
ISBN (Print)	9781493915934
DOIs	https://doi.org/10.1007/978-1-4939-1594-1_5
Publication status	Published - 6 Oct 2014

Keywords

Cell fusion
Heterokaryons
Postfusion enucleation
Reprogramming

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10.1007/978-1-4939-1594-1_5

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N2 - Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.

AB - Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.

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KW - Heterokaryons

KW - Postfusion enucleation

KW - Reprogramming

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Embryonic stem cell-somatic cell fusion and postfusion enucleation

Abstract

Keywords

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