Embryonic stem cell-somatic cell fusion and postfusion enucleation

Huseyin Sumer, Paul J. Verma

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.

Original languageEnglish
Title of host publicationNuclear Reprogramming
Subtitle of host publicationMethods and Protocols
Place of PublicationNY USA
PublisherHumana Press
Chapter5
Pages61-69
Number of pages9
Volume1222
Edition2nd
ISBN (Electronic)9781493915941
ISBN (Print)9781493915934
DOIs
Publication statusPublished - 6 Oct 2014

Keywords

  • Cell fusion
  • Heterokaryons
  • Postfusion enucleation
  • Reprogramming

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