TY - JOUR
T1 - ELISA with recombinant rKRP42 antigen using urine samples
T2 - A tool for predicting clinical visceral leishmaniasis cases and its outbreak
AU - Islam, Mohammad Zahidul
AU - Itoh, Makoto
AU - Islam, Md Anwar Ul
AU - Ekram, A. R.M.Saifuddin
AU - Rahman, Md Ajijur
AU - Takagi, Hidekazu
AU - Takesue, Atsuhide
AU - Hashiguchi, Yoshihisa
AU - Kimura, Eisaku
PY - 2012/10
Y1 - 2012/10
N2 - We reported a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) that detects immunoglobulin G (IgG) in urine using rKRP42 antigen for the diagnosis of visceral leishmaniasis (VL). The ELISA was applied to study chronological change in antibody titers in five study areas in Rajshahi district, Bangladesh. A total of 585 subjects without a past VL history were examined at least three times in the 30-month follow-up period; of these subjects, 137 (23.4%) subjects became ELISA-positive at least one time during the study. Among the positive cases, 40 (29.2%) subjects developed clinical VL, and 31 (77.5%) of these subjects showed IgG titers of ≥ 1,000 U more than one time in the study period. Considering only the first ELISA results, 22 subjects with IgG titers of ≥ 1,000 U could be found, and 21 (95.5%) of these subjects turned out to be clinical cases. The high urinary IgG titers (≥ 1,000 U) will help predict possible clinical VL cases and thus, identify an outbreak in its earlier stage.
AB - We reported a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) that detects immunoglobulin G (IgG) in urine using rKRP42 antigen for the diagnosis of visceral leishmaniasis (VL). The ELISA was applied to study chronological change in antibody titers in five study areas in Rajshahi district, Bangladesh. A total of 585 subjects without a past VL history were examined at least three times in the 30-month follow-up period; of these subjects, 137 (23.4%) subjects became ELISA-positive at least one time during the study. Among the positive cases, 40 (29.2%) subjects developed clinical VL, and 31 (77.5%) of these subjects showed IgG titers of ≥ 1,000 U more than one time in the study period. Considering only the first ELISA results, 22 subjects with IgG titers of ≥ 1,000 U could be found, and 21 (95.5%) of these subjects turned out to be clinical cases. The high urinary IgG titers (≥ 1,000 U) will help predict possible clinical VL cases and thus, identify an outbreak in its earlier stage.
UR - http://www.scopus.com/inward/record.url?scp=84867909476&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.2012.12-0168
DO - 10.4269/ajtmh.2012.12-0168
M3 - Article
C2 - 22869633
AN - SCOPUS:84867909476
SN - 0002-9637
VL - 87
SP - 658
EP - 662
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -
