Elevated Levels of 2', 5'-linked Oligoadenylate-dependent Ribonuclease L Occur as an Early Event in Colorectal Tumorigenesis

Liming Wang, Aimin Zhou, Sandip Vasavada, Beihua Dong, Huiqin Nie, James M. Church, Bryan R.G. Williams, Sipra Banerjee, Robert H. Silverman

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RNA decay in IFN-treated cells is controlled by 2', 5'-linked oligoadenylate (2-5A)-dependent RNase (RNase L), a uniquely regulated endoribonuclease that requires short 5'-phosphorylated, 2-5A for its activity. Because RNase L is also implicated in the regulation of cell proliferation, we monitored its expression in colorectal adenocarcinomas and noncancerous polyps from familial adenomatous polyposis patients. Elevated levels of RNase L mRNA and activity were found in 17 of 20 tumors compared with corresponding normal mucosa. An mAb against RNase L revealed elevated amounts of this RNase in sections of the tumors, largely in the base of the villi. The occurrence of elevated levels of RNase L seems to be an early event in colorectal tumorigenesis, suggesting that control of RNA turnover is an important step in tumor progression. These data also indicate that regulating RNase L activity may be a useful strategy in treating colorectal carcinomas.

Original languageEnglish
Pages (from-to)1421-1428
Number of pages8
JournalClinical Cancer Research
Issue number11
Publication statusPublished - 1 Nov 1995
Externally publishedYes

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