TY - JOUR
T1 - Electrospun pectin-polyhydroxybutyrate nanofibers for retinal tissue engineering
AU - Chan, Siew Yin
AU - Chan, Benjamin Qi Yu
AU - Liu, Zengping
AU - Parikh, Bhav Harshad
AU - Zhang, Kangyi
AU - Lin, Qianyu
AU - Su, Xinyi
AU - Kai, Dan
AU - Choo, Wee Sim
AU - Young, David James
AU - Oh, Xian Jun
PY - 2017/12
Y1 - 2017/12
N2 - Natural polysaccharide pectin has for the first time been grafted with polyhydroxybutyrate (PHB) via ring-opening polymerization of β-butyrolactone. This copolymer, pectin-polyhydroxybutyrate (pec-PHB), was blended with PHB in various proportions and electrospun to produce nanofibers that exhibited uniform and beadfree nanostructures, suggesting the miscibility of PHB and pec-PHB. These nanofiber blends exhibited reduced fiber diameters from 499 to 336'426 nm and water contact angles from 123.8 to 88.2 on incorporation of pec-PHB. They also displayed 39'335% enhancement of elongation at break relative to pristine PHB nanofibers. pec-PHB nanofibers were found to be noncytotoxic and biocompatible. Human retinal pigmented epithelium (ARPE-19) cells were seeded onto pristine PHB and pec-PHB nanofibers as scaffold and showed good proliferation. Higher proportions of pec-PHB (pec-PHB10 and pec-PHB20) yielded higher densities of cells with similar characteristics to normal RPE cells. We propose, therefore, that nanofibers of pec-PHB have significant potential as retinal tissue engineering scaffold materials.
AB - Natural polysaccharide pectin has for the first time been grafted with polyhydroxybutyrate (PHB) via ring-opening polymerization of β-butyrolactone. This copolymer, pectin-polyhydroxybutyrate (pec-PHB), was blended with PHB in various proportions and electrospun to produce nanofibers that exhibited uniform and beadfree nanostructures, suggesting the miscibility of PHB and pec-PHB. These nanofiber blends exhibited reduced fiber diameters from 499 to 336'426 nm and water contact angles from 123.8 to 88.2 on incorporation of pec-PHB. They also displayed 39'335% enhancement of elongation at break relative to pristine PHB nanofibers. pec-PHB nanofibers were found to be noncytotoxic and biocompatible. Human retinal pigmented epithelium (ARPE-19) cells were seeded onto pristine PHB and pec-PHB nanofibers as scaffold and showed good proliferation. Higher proportions of pec-PHB (pec-PHB10 and pec-PHB20) yielded higher densities of cells with similar characteristics to normal RPE cells. We propose, therefore, that nanofibers of pec-PHB have significant potential as retinal tissue engineering scaffold materials.
UR - http://www.scopus.com/inward/record.url?scp=85040791808&partnerID=8YFLogxK
U2 - 10.1021/acsomega.7b01604
DO - 10.1021/acsomega.7b01604
M3 - Article
C2 - 30023596
AN - SCOPUS:85040791808
SN - 2470-1343
VL - 2
SP - 8959
EP - 8968
JO - ACS Omega
JF - ACS Omega
IS - 12
ER -