Electrochemical DNA biosensors for label-free breast cancer gene marker detection

Mehmet Senel, Muamer Dervisevic, Firdevs Kokkokoğlu

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

We present an electrochemical DNA detection strategy based on self-assembled ferrocene-cored poly(amidoamine) dendrimers for the detection of a gene relevant to breast cancer. The chemisorption of three ferrocene-cored poly(amidoamine) generations and hybridization of single-stranded DNA on a Au electrode were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor demonstrated high sensitivity of 0.13 μA/(ng/ml) in the detection of the target DNA with a linear range of 1.3–20 nM and a detection limit of 0.38 nM. The DNA biosensor also has high selectivity for the target DNA, showing a clear signal difference from a noncomplementary sequence and a single-base-mismatch sequence, which was used as a model of BRAC1 gene mutation. The results shown are highly motivating for exploring DNA biosensing technology in the diagnosis of breast cancer caused by mutation of the BRAC1 gene. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)2925-2935
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number13
DOIs
Publication statusPublished - 19 May 2019

Keywords

  • Breast cancer
  • Dendrimer
  • Electrochemical DNA biosensor
  • Ferrocene
  • Poly(amidoamine)

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