Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos

Greg FitzHarris, John Carroll, Karl Swann

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple using various microinjection setups, metaphase-II mouse eggs are notoriously fragile, and nondamaging microinjection can be difficult to achieve. Here we describe a microinjection method that is based on electrophysiology, which vastly reduces microinjection damage, especially in metaphase-II eggs. When optimized, this approach allows for over 90% oocyte survival, increasing confidence in experimental results.

Original languageEnglish
Title of host publicationMitosis and Meiosis Part A
EditorsHelder Maiato, Melina Schuh
PublisherAcademic Press
Chapter19
Pages431-440
Number of pages10
ISBN (Print)9780128141441
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Cell Biology
Volume144
ISSN (Print)0091-679X

Keywords

  • Embryo
  • Microinjection
  • Mouse
  • Oocyte

Cite this

FitzHarris, G., Carroll, J., & Swann, K. (2018). Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. In H. Maiato, & M. Schuh (Eds.), Mitosis and Meiosis Part A (pp. 431-440). (Methods in Cell Biology; Vol. 144). Academic Press. https://doi.org/10.1016/bs.mcb.2018.03.036
FitzHarris, Greg ; Carroll, John ; Swann, Karl. / Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. Mitosis and Meiosis Part A. editor / Helder Maiato ; Melina Schuh. Academic Press, 2018. pp. 431-440 (Methods in Cell Biology).
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FitzHarris, G, Carroll, J & Swann, K 2018, Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. in H Maiato & M Schuh (eds), Mitosis and Meiosis Part A. Methods in Cell Biology, vol. 144, Academic Press, pp. 431-440. https://doi.org/10.1016/bs.mcb.2018.03.036

Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. / FitzHarris, Greg; Carroll, John; Swann, Karl.

Mitosis and Meiosis Part A. ed. / Helder Maiato; Melina Schuh. Academic Press, 2018. p. 431-440 (Methods in Cell Biology; Vol. 144).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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FitzHarris G, Carroll J, Swann K. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. In Maiato H, Schuh M, editors, Mitosis and Meiosis Part A. Academic Press. 2018. p. 431-440. (Methods in Cell Biology). https://doi.org/10.1016/bs.mcb.2018.03.036