Elastomeric microvalve geometry affects haemocompatibility

Crispin Szydzik, Rose J. Brazilek, Khashayar Khoshmanesh, Farzan Akbaridoust, Markus Knoerzer, Peter Thurgood, Ineke Muir, Ivan Marusic, Harshal Nandurkar, Arnan Mitchell, Warwick S. Nesbitt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This paper reports on the parameters that determine the haemocompatibility of elastomeric microvalves for blood handling in microfluidic systems. Using a comprehensive investigation of blood function, we describe a hierarchy of haemocompatibility as a function of microvalve geometry and identify a "normally-closed" v-gate pneumatic microvalve design that minimally affects blood plasma fibrinogen and von Willebrand factor composition, minimises effects on erythrocyte structure and function, and limits effects on platelet activation and aggregation, while facilitating rapid switching control for blood sample delivery. We propose that the haemodynamic profile of valve gate geometries is a significant determinant of platelet-dependent biofouling and haemocompatibility. Overall our findings suggest that modification of microvalve gate geometry and consequently haemodynamic profile can improve haemocompatibility, while minimising the requirement for chemical or protein modification of microfluidic surfaces. This biological insight and approach may be harnessed to inform future haemocompatible microfluidic valve and component design, and is an advance towards lab-on-chip automation for blood based diagnostic systems.

Original languageEnglish
Pages (from-to)1778-1792
Number of pages15
JournalLab on a Chip
Volume18
Issue number12
DOIs
Publication statusPublished - 1 Jan 2018

Cite this

Szydzik, C., Brazilek, R. J., Khoshmanesh, K., Akbaridoust, F., Knoerzer, M., Thurgood, P., ... Nesbitt, W. S. (2018). Elastomeric microvalve geometry affects haemocompatibility. Lab on a Chip, 18(12), 1778-1792. https://doi.org/10.1039/c7lc01320e
Szydzik, Crispin ; Brazilek, Rose J. ; Khoshmanesh, Khashayar ; Akbaridoust, Farzan ; Knoerzer, Markus ; Thurgood, Peter ; Muir, Ineke ; Marusic, Ivan ; Nandurkar, Harshal ; Mitchell, Arnan ; Nesbitt, Warwick S. / Elastomeric microvalve geometry affects haemocompatibility. In: Lab on a Chip. 2018 ; Vol. 18, No. 12. pp. 1778-1792.
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Szydzik, C, Brazilek, RJ, Khoshmanesh, K, Akbaridoust, F, Knoerzer, M, Thurgood, P, Muir, I, Marusic, I, Nandurkar, H, Mitchell, A & Nesbitt, WS 2018, 'Elastomeric microvalve geometry affects haemocompatibility' Lab on a Chip, vol. 18, no. 12, pp. 1778-1792. https://doi.org/10.1039/c7lc01320e

Elastomeric microvalve geometry affects haemocompatibility. / Szydzik, Crispin; Brazilek, Rose J.; Khoshmanesh, Khashayar; Akbaridoust, Farzan; Knoerzer, Markus; Thurgood, Peter; Muir, Ineke; Marusic, Ivan; Nandurkar, Harshal; Mitchell, Arnan; Nesbitt, Warwick S.

In: Lab on a Chip, Vol. 18, No. 12, 01.01.2018, p. 1778-1792.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Szydzik, Crispin

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AU - Khoshmanesh, Khashayar

AU - Akbaridoust, Farzan

AU - Knoerzer, Markus

AU - Thurgood, Peter

AU - Muir, Ineke

AU - Marusic, Ivan

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AU - Mitchell, Arnan

AU - Nesbitt, Warwick S.

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Szydzik C, Brazilek RJ, Khoshmanesh K, Akbaridoust F, Knoerzer M, Thurgood P et al. Elastomeric microvalve geometry affects haemocompatibility. Lab on a Chip. 2018 Jan 1;18(12):1778-1792. https://doi.org/10.1039/c7lc01320e