Efficient Transmission Electron Microscopy Characterization of Cell-Nanostructure Interfacial Interactions

Stella Aslanoglou, Yaping Chen, Viola Oorschot, Zlatan Trifunovic, Eric Hanssen, Koukou Suu, Nicolas H. Voelcker, Roey Elnathan

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)


Engineered nano-bio interfaces driven by tunable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Yet the interplay between substrate topography and cellular behavior is highly complex and not fully understood. A new experimental design is proposed that enables generation of ultrathin sections (lamellae) of cell-nanostructure imprints with minimal artifacts. We demonstrate the potential of such lamellae for efficient transmission electron microscopy (TEM) characterization of interfacial interactions between adherent cells and vertically aligned Si nanostructures. This approach will advance understanding of cellular responses to extracellular biophysical and biochemical cues-which is likely to facilitate the design of improved cellular manipulation technologies.

Original languageEnglish
Pages (from-to)15649-15653
Number of pages5
JournalJournal of the American Chemical Society
Issue number37
Publication statusPublished - 16 Sep 2020

Cite this