TY - JOUR
T1 - Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968
AU - Foo, Su Chern
AU - Yusoff, Fatimah Md
AU - Ismail, Maznah
AU - Basri, Mahiran
AU - Khong, Nicholas Mun Hoe
AU - Chan, Kim Wei
AU - Yau, Sook Kun
N1 - Funding Information:
The authors acknowledge the financial support of Research University Grant Scheme (RUGS) Project No. 05-02-11-1404RU (Vote No. 9300364) from Universiti Putra Malaysia and Higher Institutions Centre of Excellence (HICOE) Research Fund (Vote No. 6369100). The first author would like to express thanks to MyBrain15 PhD scholarship and Kanazawa Research Fellowship 2013 awarded by the Asian Fisheries Society. All assistance from the technical staff of Institute of Bioscience is duly acknowledged.
Publisher Copyright:
© 2015 Hainan Medical University.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2015/10
Y1 - 2015/10
N2 - Objective: To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (. C. calcitrans) extracted using six types of solvents. Methods: Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH and ABTS+ scavenging activity and iron chelating activity). Results: The methanol extract exhibited the highest yield [(22.71 ± 0.96) g/100 g dry weight (DW)], total carotenoid [(4.46 ± 0.36) mg/g DW], total phenolic [(2.49 ± 0.08) mg gallic acid equivalents/g DW] and second highest fucoxanthin content [(2.08 ± 0.03) mg fucoxanthin/g DW] as compared to other solvent extracts. Methanolic extract also exhibited significantly higher (P < 0.05) scavenging (DPPH, ABTS+) and iron chelating activities. Conclusions: Methanol was the recommended solvent for the production of antioxidant rich extract from C. calcitrans. Both carotenoids and phenolic acids were found to be positively correlated to the antioxidant capacities of C. calcitrans. Lead bioactives confirmed by subsequent high performance liquid chromatography studies were fucoxanthin, gallic acid and protocatechuic acid.
AB - Objective: To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (. C. calcitrans) extracted using six types of solvents. Methods: Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH and ABTS+ scavenging activity and iron chelating activity). Results: The methanol extract exhibited the highest yield [(22.71 ± 0.96) g/100 g dry weight (DW)], total carotenoid [(4.46 ± 0.36) mg/g DW], total phenolic [(2.49 ± 0.08) mg gallic acid equivalents/g DW] and second highest fucoxanthin content [(2.08 ± 0.03) mg fucoxanthin/g DW] as compared to other solvent extracts. Methanolic extract also exhibited significantly higher (P < 0.05) scavenging (DPPH, ABTS+) and iron chelating activities. Conclusions: Methanol was the recommended solvent for the production of antioxidant rich extract from C. calcitrans. Both carotenoids and phenolic acids were found to be positively correlated to the antioxidant capacities of C. calcitrans. Lead bioactives confirmed by subsequent high performance liquid chromatography studies were fucoxanthin, gallic acid and protocatechuic acid.
KW - Antioxidants
KW - Chaetoceros calcitrans
KW - Diatoms
KW - Efficient solvent extraction
KW - Fucoxanthin
KW - Microalgae
KW - Phenolic acids
UR - http://www.scopus.com/inward/record.url?scp=84954197820&partnerID=8YFLogxK
U2 - 10.1016/j.apjtb.2015.06.003
DO - 10.1016/j.apjtb.2015.06.003
M3 - Article
AN - SCOPUS:84954197820
SN - 2221-1691
VL - 5
SP - 834
EP - 840
JO - Asian Pacific Journal of Tropical Biomedicine
JF - Asian Pacific Journal of Tropical Biomedicine
IS - 10
ER -