Efficient ROSA26-based conditional and/or inducible transgenesis using RMCE-compatible F1 hybrid mouse embryonic stem cells

Lieven Haenebalcke, Steven Goossens, Michael Naessens, Natascha Kruse, Morvarid Farhang Ghahremani, Sonia Bartunkova, Katharina Haigh, Tim Pieters, Pieterjan Dierickx, Benjamin Drogat, Omar Nyabi, Dagmar Wirth, Jody Jonathan Haigh

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35 Citations (Scopus)


The conditional Cre/loxP system and/or the doxycycline (Dox) inducible Tet-on/off system are widely used in mouse transgenesis but often require time consuming, inefficient cloning/screening steps and extensive mouse breeding strategies. We have therefore developed a highly efficient Gateway- and recombinase-mediated cassette exchange (RMCE)-compatible system to target conditional and/or inducible constructs to the ROSA26 locus of F1 hybrid Bl6/129 ESCs, called G4 ROSALUC ESCs. By combining the Cre/loxP system with or without the inducible Tet-on system using Gateway cloning, we can rapidly generate spatial and/or temporal controllable gain-of-function constructs that can be targeted to the RMCE-compatible ROSA26 locus of the G4 ROSALUC ESCs with efficiencies close to 100 . These novel ESC-based technologies allow for the creation of multiple gain-of-function conditional and/or inducible transgenic ESC clones and mouse lines in a highly efficient and locus specific manner. Importantly, incorporating insulator sequences into the Dox-inducible vector system resulted in robust, stable transgene expression in undifferentiated ESCs but could not fully overcome transgene mosaicism in the differentiated state.
Original languageEnglish
Pages (from-to)774-785
Number of pages12
JournalStem Cell Reviews and Reports
Issue number6
Publication statusPublished - 2013

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