@inbook{d45f8dab8c24449399a803e3496adfb3,
title = "Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the piggyBac Transposon System",
abstract = "The piggyBac transposon system has been adapted to be a highly efficient genome engineering tool for transgenesis of eukaryotic cells and organisms. As with other methods of transgenesis, incorporation of an inducible promoter, such as a tetracycline-responsive element, enables inducible transgene expression. Here, we describe an efficient method of using the piggyBac system to create stably transfected mammalian cell lines, including inducible transgene expression. Gibson assembly is used to construct the required vectors as it enables multiple DNA fragments to be seamlessly assembled in a single isothermal reaction. We demonstrate an application of this approach to generate a stably transfected pluripotent stem cell line that can be induced to express a transcription factor transgene and rapidly differentiate into neurons in a single step.",
keywords = "Gibson assembly, Inducible transgene expression, Neuronal differentiation, PiggyBac, Stem cells",
author = "Jinchao Gu and Huseyin Sumer and Brett Cromer",
note = "Funding Information: This work was supported by a Tuition Fee Scholarship from Swinburne University of Technology. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2301-5\_3",
language = "English",
isbn = "9781071623008",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "49--66",
editor = "Verma, \{Paul John\} and Huseyin Sumer and Jun Liu",
booktitle = "Applications of Genome Modulation and Editing",
address = "United States of America",
}