Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 281-290 |
| Number of pages | 10 |
| Journal | Plant and Cell Physiology |
| Volume | 57 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2016 |
Keywords
- Artificial microRNA
- AUXIN RESPONSE FACTOR
- ENHANCER OF ZESTE
- Marchantia
- MicroRNA
- RESPONSE REGULATOR
- Reverse genetics
Cite this
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Efficient and inducible use of artificial microRNAs in Marchantia polymorpha. / Flores-Sandoval, Eduardo; Dierschke, Tom; Fisher, Tom J; Bowman, John L.
In: Plant and Cell Physiology, Vol. 57, No. 2, 2016, p. 281-290.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Efficient and inducible use of artificial microRNAs in Marchantia polymorpha
AU - Flores-Sandoval, Eduardo
AU - Dierschke, Tom
AU - Fisher, Tom J
AU - Bowman, John L
PY - 2016
Y1 - 2016
N2 - We describe the efficient use of artificial microRNAs (amiRs) in Marchantia polymorpha using both endogenous and heterologous primary microRNA (pri-miR) hairpin backbones. Targeting of two transcription factor genes, MpARF1 and MpRR-B, mediating different hormonal responses, demonstrated that amiRs can create specific and reproducible physiological and morphological defects, facilitating interpretation of gene function. A third amiR was designed to target a gene encoding a component of the Polycomb recessive complex 2, MpE(z), and constitutive expression of this amiR results in sporeling lethality. Adaptation of an estrogen-inducible system allowed analysis of the phenotypic effects of induction of this amiR during other stages of the life cycle. We discuss the advantages and challenges of the use of amiRs as a tool for reverse genetic analysis in M. polymorpha.
AB - We describe the efficient use of artificial microRNAs (amiRs) in Marchantia polymorpha using both endogenous and heterologous primary microRNA (pri-miR) hairpin backbones. Targeting of two transcription factor genes, MpARF1 and MpRR-B, mediating different hormonal responses, demonstrated that amiRs can create specific and reproducible physiological and morphological defects, facilitating interpretation of gene function. A third amiR was designed to target a gene encoding a component of the Polycomb recessive complex 2, MpE(z), and constitutive expression of this amiR results in sporeling lethality. Adaptation of an estrogen-inducible system allowed analysis of the phenotypic effects of induction of this amiR during other stages of the life cycle. We discuss the advantages and challenges of the use of amiRs as a tool for reverse genetic analysis in M. polymorpha.
KW - Artificial microRNA
KW - AUXIN RESPONSE FACTOR
KW - ENHANCER OF ZESTE
KW - Marchantia
KW - MicroRNA
KW - RESPONSE REGULATOR
KW - Reverse genetics
UR - http://pcp.oxfordjournals.org/content/57/2/281.full.pdf+html
U2 - 10.1093/pcp/pcv068
DO - 10.1093/pcp/pcv068
M3 - Article
VL - 57
SP - 281
EP - 290
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 2
ER -