TY - JOUR
T1 - Effects of steroids on fibroblasts
T2 - Identification of glucocorticoid-regulated proteins by two-dimensional gel electrophoresis
AU - Khalid, B. A.K.
AU - Gyorki, S.
AU - Risbridger, G. P.
AU - Warne, G. L.
AU - Funder, J. W.
PY - 1983
Y1 - 1983
N2 - Fibroblasts cultured from normal human forearm or genital skin were exposed to dexamethasone (DM) 10-9-10-7M, deoxycorticosterone (DOC) 10-6, or vehicle for 4-16 h and [-35S]methionine-labelled proteins compared by two-dimensional gel electrophoresis. No effects on cell number or [-35S]methionine incorporation were seen with either steroid, nor any effects of DOC on patterns of protein synthesis. In contrast, after 4 h incubation DM consistently (24/24 gels) caused increased abundance of a protein spot designated κ (kappa; Mr ∼ 41000, pKi, ∼ 6.5). After 16 h steroid exposure, levels of protein κ remained elevated; abundance of a second protein spot ω (omega; Mr ∼ 42000, pK1 ∼5.4) was consistently (8/8 gels) lower than in control fibroblasts. We interpret these data as evidence for glucocorticoid-specific effects on human skin fibroblasts, and that (within the pK1 and molecular weight range studied) the observed changes in abundance of proteins κ and ω constitute the human fibroblast glucocorticoid domain.
AB - Fibroblasts cultured from normal human forearm or genital skin were exposed to dexamethasone (DM) 10-9-10-7M, deoxycorticosterone (DOC) 10-6, or vehicle for 4-16 h and [-35S]methionine-labelled proteins compared by two-dimensional gel electrophoresis. No effects on cell number or [-35S]methionine incorporation were seen with either steroid, nor any effects of DOC on patterns of protein synthesis. In contrast, after 4 h incubation DM consistently (24/24 gels) caused increased abundance of a protein spot designated κ (kappa; Mr ∼ 41000, pKi, ∼ 6.5). After 16 h steroid exposure, levels of protein κ remained elevated; abundance of a second protein spot ω (omega; Mr ∼ 42000, pK1 ∼5.4) was consistently (8/8 gels) lower than in control fibroblasts. We interpret these data as evidence for glucocorticoid-specific effects on human skin fibroblasts, and that (within the pK1 and molecular weight range studied) the observed changes in abundance of proteins κ and ω constitute the human fibroblast glucocorticoid domain.
KW - fibroblasts
KW - glucocorticoids
KW - two-dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0020537254&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(83)90066-7
DO - 10.1016/0303-7207(83)90066-7
M3 - Article
C2 - 6862096
AN - SCOPUS:0020537254
SN - 0303-7207
VL - 30
SP - 303
EP - 312
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 3
ER -