Effects of quantum dots in polymerase chain reaction

Libing  Wang; Yingyue Zhu; Yuan Jiang; Ruirui Qiao; Shuifang Zhu; Wei Chen; Chuanlai  Xu

doi:10.1021/jp902404y

Effects of quantum dots in polymerase chain reaction

Libing Wang
, Yingyue Zhu
, Yuan Jiang
, Ruirui Qiao
, Shuifang Zhu
, Wei Chen
, Chuanlai Xu

Research output: Contribution to journal › Article › Research › peer-review

59 Citations (Scopus)

Abstract

The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results snowed that there was no enhancement. The mechanisms underlying these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.

Original language	English
Pages (from-to)	7637-7641
Number of pages	5
Journal	Journal of Physical Chemistry B
Volume	113
Issue number	21
DOIs	https://doi.org/10.1021/jp902404y
Publication status	Published - 28 May 2009
Externally published	Yes

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title = "Effects of quantum dots in polymerase chain reaction",

abstract = "The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results snowed that there was no enhancement. The mechanisms underlying these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.",

author = "Libing Wang and Yingyue Zhu and Yuan Jiang and Ruirui Qiao and Shuifang Zhu and Wei Chen and Chuanlai Xu",

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T1 - Effects of quantum dots in polymerase chain reaction

AU - Wang, Libing

AU - Zhu, Yingyue

AU - Jiang, Yuan

AU - Qiao, Ruirui

AU - Zhu, Shuifang

AU - Chen, Wei

AU - Xu, Chuanlai

PY - 2009/5/28

Y1 - 2009/5/28

N2 - The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results snowed that there was no enhancement. The mechanisms underlying these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.

AB - The effects of quantum dots (QDs) on the elimination of nonspecific amplification of the polymerase chain reaction (PCR) were investigated. It was found that QDs could increase the specificity of the PCR at different annealing temperatures and with DNA templates of different lengths. The effects of QDs on the efficiency of the PCR were also studied, and the results snowed that there was no enhancement. The mechanisms underlying these effects are discussed. This method could be used to modify the amplification results of the conventional PCR. Furthermore, this technology could make the PCR more widely applicable, especially in the multi-PCR reaction system with different annealing temperatures. This is of great significance for gene diagnosis.

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JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

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ER -

Effects of quantum dots in polymerase chain reaction

Abstract

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