Effects of preterm birth induced with or without exogenous glucocorticoids on the ovine glucose-insulin axis

Amita Bansal, Jane M. Alsweiler, Mark H. Oliver, Anne Jaquiery, Hui Hui Phua, Mike Dragunow, Rob de Matteo, Jane E. Harding, Frank H. Bloomfield

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Abstract

Antenatal exogenous glucocorticoids (ANG) are standard management for women at risk of preterm birth but are reputed to impair glucose tolerance in preterm offspring. We compared lambs born preterm (137 days gestation) following labour induced with exogenous glucocorticoids (G-Prem, glucocorticoid-induced preterm group), or with a progesterone synthesis inhibitor (NG-Prem, non-glucocorticoid-induced preterm group), with term-born lambs (Term; 149 days). We assessed glucose tolerance, insulin secretion and sensitivity at 4 and 10 months n = 11-14/group) and pancreatic and hepatic gene and protein expression at 4 weeks post-term (4 weeks; n = 6/group) and 12 months (12 months; n = 12-13/group). NG-Prem had higher plasma glucose concentrations than G-Prem, but not Term, at 4 months (Mean[SEM] mM: NG-Prem = 4.1[0.1]; G-Prem = 3.4[0.1]; Term = 3.7[0.1]; p = 0.003) and 10 months (NG-Prem = 3.9[0.1]; G-Prem = 3.5[0.1]; Term = 3.7[0.1]; p = 0.01). Insulin sensitivity decreased from 4 to 10 months, in NG-Prem but not in Term (Mean[SEM] μmol·ml-1·kg-1·min-1·ng-1, 4 vs. 10 months: NG-Prem = 18.7[2.5] vs. 9.5[1.5], p < 0.01; Term: 12.1[2.8] vs. 10.4[1.5], p = 0.44). At 12 months, β-cell mass in NG-Prem was reduced by 30% vs. G-Prem (p < 0.01) and 75% vs. Term (p < 0.01) and was accompanied by an increased β-cell apoptosis: Proliferation ratio at 12 months. At 12 months, pancreatic glucokinase, igf2 and insulin mRNA levels were reduced 21%-71% in NG-Prem vs. G-Prem and 42%-80% vs. Term. Hepatic glut2 mRNA levels in NG-Prem were 250% of those in G-Prem and Term. Thus, induction of preterm birth without exogenous glucocorticoids more adversely affected pancreas and liver than induction with exogenous glucocorticoids. These findings do not support that ANG lead to long-term adverse metabolic effects, but support an effect of preterm birth itself.

Original languageEnglish
Number of pages13
JournalJournal of Developmental Origins of Health and Disease
DOIs
Publication statusAccepted/In press - 15 Jan 2020

Keywords

  • insulin secretion
  • insulin sensitivity
  • Preterm birth
  • steroids
  • β-cells

Cite this

Bansal, A., Alsweiler, J. M., Oliver, M. H., Jaquiery, A., Phua, H. H., Dragunow, M., de Matteo, R., Harding, J. E., & Bloomfield, F. H. (Accepted/In press). Effects of preterm birth induced with or without exogenous glucocorticoids on the ovine glucose-insulin axis. Journal of Developmental Origins of Health and Disease. https://doi.org/10.1017/S2040174419000916