Effects of insulin-like growth factor binding protein complexes on human fibroblast growth

G. H. Tesch, H. J. Cornell, A. C. Herington, S. Oakes

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Insulin-like growth factor binding protein (IGF-BP) complexes containing either IGF-I or IGF-II were purified from human plasma and coincubated with human foreskin fibroblasts. Incorporation of [3H]thymidine into fibroblasts was measured to determine the mitogenic effects of the IGF-BP complexes in comparison with purified recombinant IGF-I or IGF-II. The IGF-I-BP complex was shown to have no stimulatory activity when added to cultures at a comparable Non Suppressible Insulin-Like Activity (NSILA) dose level to that of purified recombinant IGF-I which produced maximal stimulation. In contrast, the IGF-II-BP complex produced a similar stimulatory activity to that of purified recombinant IGF-II when added at comparable NSILA doses. NSILA-p, a contaminating factor in the purified preparations of IGF-BP complexes, was shown to have no effect on fibroblast growth. This study suggests that plasma IGF binding proteins may have a differential effect on the stimulatory activities of IGFs I and II on human fibroblasts.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalGrowth Regulation
Issue number2
Publication statusPublished - 1 Jan 1993
Externally publishedYes


  • Binding proteins
  • Fibroblasts
  • Insulin-like growth factors

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