Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching

Wei Chen; Shenghua Yin; I.M.S.K. Ilankoon

doi:10.1007/s12613-020-2125-x

Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching

Wei Chen
, Shenghua Yin
, I.M.S.K. Ilankoon

Malaysia School of Engineering

Research output: Contribution to journal › Article › Research › peer-review

29 Citations (Scopus)

Abstract

Bacterial community dynamics and copper leaching with applied forced aeration were investigated during low-grade copper sulphide bioleaching to obtain better bioleaching efficiency. Results illustrated that appropriate aeration improved bacterial concentrations and leaching efficiencies. The highest bacterial concentration and Cu²⁺ concentration after 14-d leaching were 7.61 × 10⁷ cells·mL⁻¹ and 704.9 mg·L⁻¹, respectively, at aeration duration of 4 h·d⁻¹. The attached bacteria played a significant role during bioleaching from 1 to 7 d. However, free bacteria dominated the bioleaching processes from 8 to 14 d. This phenomenon was mainly caused by the formation of passivation layer through Fe³⁺ hydrolysis along with bioleaching, which inhibited the contact between the attached bacteria and ore. Meanwhile, 16S rDNA analysis verified the effect of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans on the bioleaching process. The results demonstrate the importance of free and attached bacteria in bioleaching.

Original language	English
Pages (from-to)	59-69
Number of pages	11
Journal	International Journal of Minerals, Metallurgy, and Materials
Volume	29
Issue number	1
DOIs	https://doi.org/10.1007/s12613-020-2125-x
Publication status	Published - Jan 2022

Keywords

attached bacteria
bioleaching
forced aeration
free bacteria
low-grade copper sulphide ore

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title = "Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching",

abstract = "Bacterial community dynamics and copper leaching with applied forced aeration were investigated during low-grade copper sulphide bioleaching to obtain better bioleaching efficiency. Results illustrated that appropriate aeration improved bacterial concentrations and leaching efficiencies. The highest bacterial concentration and Cu2+ concentration after 14-d leaching were 7.61 × 107 cells·mL−1 and 704.9 mg·L−1, respectively, at aeration duration of 4 h·d−1. The attached bacteria played a significant role during bioleaching from 1 to 7 d. However, free bacteria dominated the bioleaching processes from 8 to 14 d. This phenomenon was mainly caused by the formation of passivation layer through Fe3+ hydrolysis along with bioleaching, which inhibited the contact between the attached bacteria and ore. Meanwhile, 16S rDNA analysis verified the effect of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans on the bioleaching process. The results demonstrate the importance of free and attached bacteria in bioleaching.",

keywords = "attached bacteria, bioleaching, forced aeration, free bacteria, low-grade copper sulphide ore",

author = "Wei Chen and Shenghua Yin and I.M.S.K. Ilankoon",

note = "Funding Information: This work was supported by National Science Foundation for Excellent Young Scholars, China (No. 51722401), Key Project of National Natural Science Foundation, China (No. 51734001), and Fundamental Research Funds for the Central Universities (No. FRF-TP-18-003C1). Publisher Copyright: {\textcopyright} 2022, University of Science and Technology Beijing.",

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doi = "10.1007/s12613-020-2125-x",

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T1 - Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching

AU - Chen, Wei

AU - Yin, Shenghua

AU - Ilankoon, I.M.S.K.

N1 - Funding Information: This work was supported by National Science Foundation for Excellent Young Scholars, China (No. 51722401), Key Project of National Natural Science Foundation, China (No. 51734001), and Fundamental Research Funds for the Central Universities (No. FRF-TP-18-003C1). Publisher Copyright: © 2022, University of Science and Technology Beijing.

PY - 2022/1

Y1 - 2022/1

N2 - Bacterial community dynamics and copper leaching with applied forced aeration were investigated during low-grade copper sulphide bioleaching to obtain better bioleaching efficiency. Results illustrated that appropriate aeration improved bacterial concentrations and leaching efficiencies. The highest bacterial concentration and Cu2+ concentration after 14-d leaching were 7.61 × 107 cells·mL−1 and 704.9 mg·L−1, respectively, at aeration duration of 4 h·d−1. The attached bacteria played a significant role during bioleaching from 1 to 7 d. However, free bacteria dominated the bioleaching processes from 8 to 14 d. This phenomenon was mainly caused by the formation of passivation layer through Fe3+ hydrolysis along with bioleaching, which inhibited the contact between the attached bacteria and ore. Meanwhile, 16S rDNA analysis verified the effect of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans on the bioleaching process. The results demonstrate the importance of free and attached bacteria in bioleaching.

AB - Bacterial community dynamics and copper leaching with applied forced aeration were investigated during low-grade copper sulphide bioleaching to obtain better bioleaching efficiency. Results illustrated that appropriate aeration improved bacterial concentrations and leaching efficiencies. The highest bacterial concentration and Cu2+ concentration after 14-d leaching were 7.61 × 107 cells·mL−1 and 704.9 mg·L−1, respectively, at aeration duration of 4 h·d−1. The attached bacteria played a significant role during bioleaching from 1 to 7 d. However, free bacteria dominated the bioleaching processes from 8 to 14 d. This phenomenon was mainly caused by the formation of passivation layer through Fe3+ hydrolysis along with bioleaching, which inhibited the contact between the attached bacteria and ore. Meanwhile, 16S rDNA analysis verified the effect of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans on the bioleaching process. The results demonstrate the importance of free and attached bacteria in bioleaching.

KW - attached bacteria

KW - bioleaching

KW - forced aeration

KW - free bacteria

KW - low-grade copper sulphide ore

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JF - International Journal of Minerals, Metallurgy, and Materials

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Effects of forced aeration on community dynamics of free and attached bacteria in copper sulphide ore bioleaching

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