Effects of differing oocyte-secreted factors during mouse in vitro maturation on subsequent embryo and fetal development

J. Sudiman, Lesley J Ritter, D. K. Feil, X. Wang, K. Chan, David G Mottershead, D. M. Robertson, J. G. Thompson, Robert B. Gilchrist

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

Purpose: We hypothesised that varying native oocyte-secreted factor (OSF) exposure or using different recombinant OSF peptides would have differential effects on post-in vitro maturation (IVM) embryo and fetal development. Methods: Mouse cumulus oocyte complexes (COCs) were treated with the purified mature domain of GDF9 and/or BMP15 or were co-cultured with denuded oocytes (DOs) from 0 h or 3 h of IVM. DOs were matured for 3 h as either intact COCs+/-FSH before denuding, or as DOs + FSH. COCs were fertilised and blastocyst development was assessed on days 5 and 6, and either differentially stained for ICM numbers or vitrified/warmed embryos were transferred to recipients to assess implantation and fetal rates. Results: No improvement in embryo development was observed with the addition of GDF9 and/or BMP15 to IVM. In contrast, embryos derived from COCs co-cultured with DOs had significantly improved blastocyst rates and ICM numbers compared to controls (P < 0.05). The highest response was obtained when DOs were first added to COCs at 3 h of IVM, after being pre-treated (0-3 h) as COCs + FSH. Compared to control, co-culture with DOs from 3 h did not affect implantation rates but more than doubled fetal yield (21 % vs 48 %; P < 0.05). GDF9 Western blot analysis was unable to detect any differences in quantity or form of GDF9 (17 and 65 kDa) in extracts of DO at 0 h or 3 h. Conclusions: This study provides new knowledge on means to improve oocyte quality in vitro which has the potential to significantly aid human infertility treatment and animal embryo production technologies.

Original languageEnglish
Pages (from-to)295-306
Number of pages12
JournalJournal of Assisted Reproduction and Genetics
Volume31
Issue number3
DOIs
Publication statusPublished - Mar 2014
Externally publishedYes

Keywords

  • BMP15
  • GDF9
  • Oocyte in vitro maturation (IVM)
  • Oocyte-secreted factor (OSFs)

Cite this