TY - JOUR
T1 - Effects of amylin deficiency on trabecular bone in young mice are sex-dependent
AU - Davey, Rachel
AU - Moore, Alison
AU - Chiu, W S Maria
AU - Notini, Amanda
AU - Morris, Howard
AU - Zajac, Jeffrey
PY - 2006
Y1 - 2006
N2 - Amylin deficiency in mice results in late-onset osteopenia. Sex differences have been identified in insulin secretion in Amylin-overexpressing transgenic mice, suggesting a possible interaction of sex steroids, growth factors, or cytokines and amylin. The aim of the current study was to compare the effects of amylin deficiency on bone in young and adult male and female mice. The metaphyses of the distal femora from male and female Amylin-deficient mice at 4, 6, and 26 weeks of age were assessed by bone histomorphometry. Femoral length was increased in Amylin-deficient male mice compared to wild-type (WT) mice at 26 weeks of age (P <0.005) but not in females. This was associated with an increase in growth plate height in Amylin-deficient males at 4 (P <0.01) and 6 (P <0.05) weeks of age. Furthermore, young Amylin-deficient males had decreased trabecular number at 4 weeks of age (P <0.05) and increased trabecular thickness at 4 and 6 weeks of age (P <0.05) compared to WT mice, with no net change in trabecular bone volume. These effects of amylin deficiency were not observed in female mice. In conclusion, this study demonstrates that amylin deficiency exerts effects on bone during growth that are sex-dependent and suggest a possible interaction between amylin and testosterone, growth factors, or cytokines to regulate bone cell metabolism.
AB - Amylin deficiency in mice results in late-onset osteopenia. Sex differences have been identified in insulin secretion in Amylin-overexpressing transgenic mice, suggesting a possible interaction of sex steroids, growth factors, or cytokines and amylin. The aim of the current study was to compare the effects of amylin deficiency on bone in young and adult male and female mice. The metaphyses of the distal femora from male and female Amylin-deficient mice at 4, 6, and 26 weeks of age were assessed by bone histomorphometry. Femoral length was increased in Amylin-deficient male mice compared to wild-type (WT) mice at 26 weeks of age (P <0.005) but not in females. This was associated with an increase in growth plate height in Amylin-deficient males at 4 (P <0.01) and 6 (P <0.05) weeks of age. Furthermore, young Amylin-deficient males had decreased trabecular number at 4 weeks of age (P <0.05) and increased trabecular thickness at 4 and 6 weeks of age (P <0.05) compared to WT mice, with no net change in trabecular bone volume. These effects of amylin deficiency were not observed in female mice. In conclusion, this study demonstrates that amylin deficiency exerts effects on bone during growth that are sex-dependent and suggest a possible interaction between amylin and testosterone, growth factors, or cytokines to regulate bone cell metabolism.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16830202
U2 - 10.1007/s00223-005-0286-2
DO - 10.1007/s00223-005-0286-2
M3 - Article
VL - 78
SP - 398
EP - 403
JO - Calcified Tissue International
JF - Calcified Tissue International
SN - 0171-967X
IS - 6
ER -