Effects of 2-hydroxyoestradiol-17β on plasma luteinizing hormone, follicle-stimulating hormone and prolactin, and nuclear translocation of pituitary oestrogen receptors in ovariectomized ewes

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Abstract

The ability of oestradiol-17β (OE2) and 2-hydroxyoestradiol (2OH-OE2) to translocate pituitary oestrogen receptors to the nuclear compartment and to affect plasma concentrations of LH, FSH and prolactin was studied in ovariectomized ewes. Mean (± S.E.M.) nuclear oestrogen receptor levels (% of total pituitary oestrogen receptors) after intracarotid injections of 1.25 μg OE2, 400 μg 2OH-OE2 or vehicle were 8.7 ± 2.3, 16.9 ± 3.2 and 0.8 ± 0.1% respectively. Whereas 1.25 or 12.5 μg OE2 significantly lowered plasma LH and FSH, 400 μg 2OH-OE2 did not affect plasma LH or FSH levels. Injection of 4000 μg 2OH-OE2, however, significantly affected plasma LH and FSH. Plasma prolactin levels were not significantly affected by the treatments. These data indicate a discrepancy between oestrogen receptor occupancy and effects on gonadotrophin and prolactin secretion after injection of catechol oestrogen.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalJournal of Endocrinology
Volume95
Issue number1
DOIs
Publication statusPublished - 1 Jan 1982
Externally publishedYes

Cite this

@article{02ccda422e7c465d9da4496874632100,
title = "Effects of 2-hydroxyoestradiol-17β on plasma luteinizing hormone, follicle-stimulating hormone and prolactin, and nuclear translocation of pituitary oestrogen receptors in ovariectomized ewes",
abstract = "The ability of oestradiol-17β (OE2) and 2-hydroxyoestradiol (2OH-OE2) to translocate pituitary oestrogen receptors to the nuclear compartment and to affect plasma concentrations of LH, FSH and prolactin was studied in ovariectomized ewes. Mean (± S.E.M.) nuclear oestrogen receptor levels ({\%} of total pituitary oestrogen receptors) after intracarotid injections of 1.25 μg OE2, 400 μg 2OH-OE2 or vehicle were 8.7 ± 2.3, 16.9 ± 3.2 and 0.8 ± 0.1{\%} respectively. Whereas 1.25 or 12.5 μg OE2 significantly lowered plasma LH and FSH, 400 μg 2OH-OE2 did not affect plasma LH or FSH levels. Injection of 4000 μg 2OH-OE2, however, significantly affected plasma LH and FSH. Plasma prolactin levels were not significantly affected by the treatments. These data indicate a discrepancy between oestrogen receptor occupancy and effects on gonadotrophin and prolactin secretion after injection of catechol oestrogen.",
author = "Clarke, {I. J.} and K. Wynne and Funder, {J. W.} and Findlay, {J. K.}",
year = "1982",
month = "1",
day = "1",
doi = "10.1677/joe.0.0950019",
language = "English",
volume = "95",
pages = "19--27",
journal = "Journal of Endocrinology",
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TY - JOUR

T1 - Effects of 2-hydroxyoestradiol-17β on plasma luteinizing hormone, follicle-stimulating hormone and prolactin, and nuclear translocation of pituitary oestrogen receptors in ovariectomized ewes

AU - Clarke, I. J.

AU - Wynne, K.

AU - Funder, J. W.

AU - Findlay, J. K.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The ability of oestradiol-17β (OE2) and 2-hydroxyoestradiol (2OH-OE2) to translocate pituitary oestrogen receptors to the nuclear compartment and to affect plasma concentrations of LH, FSH and prolactin was studied in ovariectomized ewes. Mean (± S.E.M.) nuclear oestrogen receptor levels (% of total pituitary oestrogen receptors) after intracarotid injections of 1.25 μg OE2, 400 μg 2OH-OE2 or vehicle were 8.7 ± 2.3, 16.9 ± 3.2 and 0.8 ± 0.1% respectively. Whereas 1.25 or 12.5 μg OE2 significantly lowered plasma LH and FSH, 400 μg 2OH-OE2 did not affect plasma LH or FSH levels. Injection of 4000 μg 2OH-OE2, however, significantly affected plasma LH and FSH. Plasma prolactin levels were not significantly affected by the treatments. These data indicate a discrepancy between oestrogen receptor occupancy and effects on gonadotrophin and prolactin secretion after injection of catechol oestrogen.

AB - The ability of oestradiol-17β (OE2) and 2-hydroxyoestradiol (2OH-OE2) to translocate pituitary oestrogen receptors to the nuclear compartment and to affect plasma concentrations of LH, FSH and prolactin was studied in ovariectomized ewes. Mean (± S.E.M.) nuclear oestrogen receptor levels (% of total pituitary oestrogen receptors) after intracarotid injections of 1.25 μg OE2, 400 μg 2OH-OE2 or vehicle were 8.7 ± 2.3, 16.9 ± 3.2 and 0.8 ± 0.1% respectively. Whereas 1.25 or 12.5 μg OE2 significantly lowered plasma LH and FSH, 400 μg 2OH-OE2 did not affect plasma LH or FSH levels. Injection of 4000 μg 2OH-OE2, however, significantly affected plasma LH and FSH. Plasma prolactin levels were not significantly affected by the treatments. These data indicate a discrepancy between oestrogen receptor occupancy and effects on gonadotrophin and prolactin secretion after injection of catechol oestrogen.

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U2 - 10.1677/joe.0.0950019

DO - 10.1677/joe.0.0950019

M3 - Article

VL - 95

SP - 19

EP - 27

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

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