Effects of 17 alpha-ethinyl estradiol exposure on estrogen receptors alpha and beta and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Joseph L Humble, Minna Mari Saaristo, Kai Lindstrom, Kari K Lehtonen, John A Craft

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13 Citations (Scopus)

Abstract

This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with erα was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days. All four transcripts were significantly induced due to exposure and expression rose during the time course with distinct temporal patterns and vtga reached a substantially higher level at the end of the time course coinciding with rapid elevation in erα expression.
Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalMarine Environmental Research
Volume96
DOIs
Publication statusPublished - 2014
Externally publishedYes

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