Effective purification of lysozyme from chicken egg white by tris(hydroxymethyl)aminomethane affinity nanofiber membrane

Bing Lan Liu, Chien Wei Ooi, I. Son Ng, Pau Loke Show, Kai Jie Lin, Yu Kaung Chang

    Research output: Contribution to journalArticleResearchpeer-review

    7 Citations (Scopus)

    Abstract

    Polyacrylonitrile nanofiber membrane functionalized with tris(hydroxymethyl)aminomethane (P-Tris) was used in affinity membrane chromatography for lysozyme adsorption. The effects of pH and protein concentration on lysozyme adsorption were investigated. Based on Langmuir model, the adsorption capacity of P-Tris nanofiber membrane was estimated to be 345.83 mg/g. For the operation of dynamic membrane chromatography with three-layer P-Tris nanofiber membranes, the optimal operating conditions were at pH 9, 1.0 mL/min of feed flow rate, and 2 mg/mL of feed concentration. Chicken egg white (CEW) was applied as the crude feedstock of lysozyme in the optimized dynamic membrane chromatography. The percent recovery and purification factor of lysozyme obtained from the chromatography were 93.28% and 103.98 folds, respectively. Our findings demonstrated the effectiveness of P-Tris affinity nanofiber membrane for the recovery of lysozyme from complex CEW solution.

    Original languageEnglish
    Article number127038
    Number of pages12
    JournalFood Chemistry
    Volume327
    DOIs
    Publication statusPublished - 15 Oct 2020

    Keywords

    • Affinity nanofiber membrane
    • Chicken egg white
    • Lysozyme purification
    • Membrane chromatography
    • Tris(hydroxymethyl)aminomethane

    Cite this